merck millipore,默克密理博,MAB326A4,Anti-CNPase Antibody, clone 11-5B, Alexa Fluor488 Conjugate

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
Sh, R, H, M, Rb, Po, Ca, B	ICC	M	AlexaFluor®488	Monoclonal Antibody

描述
产品目录编号	MAB326A4
描述	Anti-CNPase Antibody, clone 11-5B, Alexa Fluor®488 Conjugate
Alternate Names	CNPase 2',3'-cyclic-nucleotide 3'-phosphodiesterase CNP
背景信息	CNPase (2', 3'-cyclic nucleotide 3'-phosphodiesterase [or -phosphohydrolase], EC 3.1.4.37) is present in very high levels in brain and peripheral nerve. This enzyme is found almost exclusively in oligodendrocytes and Schwann cells, the cells that form myelin in the central and peripheral nervous system, respectively. Immunohistochemical localization of CNPase has shown the enzyme to be restricted to oligodendrocytes and Schwann cells. The enzyme appears to be distributed in single and double loose wraps of myelin and not in compact myelin as earlier thought by most investigators. CNPase is located on the inner and outer loops of myelin, paranodally and near the inner surface of the oligodendrocyte membrane. In plaque regions of multiple sclerosis patients, the enzyme is reduced, and when CNS myelin is decreased, CNPase is one of the earlier proteins to be lost from the myelin. In addition, an enzyme that is probably identical to brain CNPase is located in the outer rod segments within the visual system, and this protein is also recognized by the monoclonal antibody 11-5B. In mixed human gliomas, the enzyme levels are reduced, although about 5% of the oligodendrocytes occasionally show normal positive staining.

产品信息
格式	AlexaFluor®488
控制	Rat oligodendrocyte precursor cells
演示	Purified mouse monoclonal IgG1 conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

应用
应用	Anti-CNPase Antibody, clone 11-5B, Alexa Fluor®488 Conjugate detects level of CNPase & has been published & validated for use in ICC.
主要应用	Immunocytochemistry

生物信息
免疫原品种	Purified human brain CNPase
克隆	11-5B
宿主	Mouse
特异性	The unconjugated parent antibody (Cat. No. MAB326) reacts with CNPase, 48, and 46 kDa polypeptides by SDS-PAGE. Differentiates clearly oligodendrocytes and Schwann cells from neurons and astrocytes.
同种型	IgG1
品种反应性	SheepRatHumanMouseRabbitPigCanineBovine
Species Reactivity Note	Demonstrated to react with Rat. Predicted to react with Human, Mouse, Rabbit, Porcine, Canine, Bovine, and Sheep based on 100% sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_149124
基因符号	CNP
纯化方法	Protein A purified
UniProt编号	P09543
UniProt汇总	SIZE: 421 amino acids; 47579 Da SUBCELLULAR LOCATION: Membrane. Melanosome. Note=Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. SIMILARITY: Belongs to the cyclic nucleotide phosphodiesterase family.

产品使用声明
质量保证	Evaluated by Immunocytochemistry in rat oligodendrocyte precursor cells. Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected CNPase in rat oligodendrocyte precursor cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

包装信息
数量	100 µL

merck millipore,默克密理博,MAB326A4,Anti-CNPase Antibody, clone 11-5B, Alexa Fluor488 Conjugate

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