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品种反应性 主要应用 宿主 抗体类型 R, H, M WB Rb Polyclonal Antibody 描述 产品目录编号 07-1383 描述 Anti-CDT1 Antibody Alternate Names - Double parked, Drosophila, homolog of 2 chromatin licensing and DNA replication factor 1 <br />
- DNA replication factor
- Double parked homolog
背景信息 CDT1 (Chromatin Licensing and DNA replication Factor 1) is an essential component of the cell cycle machinery. It plays an important role in insuring that there is only one round of DNA replication per cell cycle. CDT1 is a known substrate of Cdk2 and Cdk4 (Liu, E. 2004). It is presented in G1 of the cell cycle and is degraded as cells enter S phase (Liu, E. 2004). In higher eukaryotes, CDT1 is known to interact with Geminin, a protein that is degraded during mitosis, that inhibits it. CDT1 is marked for degradation by two E3 ligases: SCF complex and the CUL4-DDB1 complex (Sansam, 2006). 产品信息 演示 Unpurified rabbit serum containing 0.05% NaN3. 应用 应用 Detect CDT1 using this Anti-CDT1 Antibody validated for use in WB & IP. 主要应用 - Western Blotting
应用说明 An independent lab detected this antibody to immunoprecipitate (IP) Cdt1 using 2 μL/IP reaction (Wohlschlegel, B.T., 2000). 生物信息 免疫原品种 Recombinant protein including amino acids 238-546 of human CDT1. 表位 C-terminal half 宿主 Rabbit 特异性 This antibody recognizes the Cdt1. 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse, and Rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_112190.2
基因符号 - RIS2
- DUP
UniProt编号 - Q9H211
UniProt汇总 FUNCTION: Cooperates with CDC6 to promote the loading of the mini-chromosome maintenance complex onto chromatin to form the pre-replication complex necessary to initiate DNA replication. Binds DNA in a sequence-, strand-, and conformation-independent manner. Potential oncogene. UniProtKB Q8R4E9
SUBUNIT STRUCTURE: Interaction with GMNN inhibits both binding of the MCM complex to origins of replication and DNA-binding activity. UniProtKB Q8R4E9
SUBCELLULAR LOCATION: Nucleus.
DEVELOPMENTAL STAGE: Present during G1 and early S phase of the cell cycle. Degraded during the late S, G2, and M phases.
PTM: Ubiquitinated, leading to its subsequent degradation. The interaction with GMNN protects it against ubiquitination.
Phosphorylated by cyclin A-dependent kinases which results in the binding of CDT1 to the F-box protein SKP2 and subsequent degradation. Binding to GMNN is not affected by phosphorylation.
SEQUENCE SIMILARITIES: Belongs to the Cdt1 family.
SEQUENCE CAUTION: The sequence AF070552 differs from that shown. Reason: Frameshift at positions 278 and 312.
产品使用声明 质量保证 Evaluated by western blot on Jurkat cell lysate.
Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect CDT1 in Jurkat cell lysate.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 2 years at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
包装信息 数量 100 µL
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