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Replacement Information Replacement Information 07-1229 is a recommended replacement for AB3606. 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1229 Replaces AB3606 描述 Anti-DAPK2 Antibody Alternate Names - Death-associated protein kinase
- DAP kinase
- DAP- kinase-related protein
背景信息 Death Associated Protein Kinase (DAPK) is a Ca2+/Calmodulin-dependent, cytoskeletal associated protein kinase whose expression is implicated in the sensitivity of cells to apoptotic effects of TNFα and Interferon-γ. Deletion of the Calmodulin-binding domain results in a constitutively active mutant with a more potent cytotoxic effect. In contrast, the catalytically inactive mutant exerts a dominant negative effect, reducing cytotoxicity and protecting cells from Interferon-γ induced cell death. DAPK expression is frequently lost in human carcinomas and B-cell leukemia, and lower levels of expression correlate with high rates of metastasis. 产品信息 格式 Affinity Purified 控制 - C2C12 cell lysate
演示 Purified rabbit polyclonal IgG in buffer containing PBS and 0.02% sodium azide. 应用 应用 Detect DAPK2 using this Anti-DAPK2 Antibody validated for use in WB, IH(P). 主要应用 - Western Blotting
应用说明 Immunohistochemisty (paraffin): DAPK2 representative staining pattern/morphology in apoptotic bodies in colon cancer. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.
Western Blot: A previous lot of this antibody was tested on multiple lysates. See datasheet.生物信息 免疫原品种 Peptide corresponding to aa 356-370 (ARRKALHPRRRSSTS) of human DAPK2, which is identical to the sequence of mouse origin. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Detects a 42 kDa band by western blot, corresponding to DAPK2. Does not cross-react with DAPK1. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human, Mouse and Rat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_014326
Entrez基因汇总 This gene encodes a protein that belongs to the serine/threonine protein kinase family. This protein contains a N-terminal protein kinase domain followed by a conserved calmodulin-binding domain with significant similarity to that of death-associated protein kinase 1 (DAPK1), a positive regulator of programmed cell death. Overexpression of this gene was shown to induce cell apoptosis. It uses multiple polyadenylation sites. 基因符号 - EC 2.7.11.1
- DAPK2
- DRP-1
- MGC119312
纯化方法 Affinity Purfied UniProt编号 - Q9UIK4
UniProt汇总 FUNCTION: SwissProt: Q9UIK4 # Calcium/calmodulin-dependent serine/threonine kinase which acts as a positive regulator of apoptosis.
COFACTOR: Magnesium.
SIZE: 370 amino acids; 42898 Da
SUBUNIT: Homodimer. Homodimerization is required for apoptotic function and is inhibited by autophosphorylation at Ser-318.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitously expressed in all tissue types examined. High levels in heart, lung and skeletal muscle.
SIMILARITY: SwissProt: Q9UIK4 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. & Contains 1 protein kinase domain. ...产品使用声明 质量保证 Routinely evaluated by Western Blot on C2C12 lysates.
Western Blot Analysis:
1:500-1,1000 dilution of this lot detected DAPK2 on 10 μg of C2C12 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at 2-8° C from date of receiept. 包装信息 数量 100 µg
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