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merck millipore,默克密理博,ECM504,QCM Chemotaxis Cell Migration Assay, 24-well (3µm), colorimetric
产品名称：QCM Chemotaxis Cell Migration Assay, 24-well (3µm), colorimetric
产品型号：ECM504
This 3 µm QCM Chemotaxis Assay 24-well-colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle.
merck millipore,默克密理博,ECM504,QCM Chemotaxis Cell Migration Assay, 24-well (3µm), colorimetric
- 产品介绍
重要规格表
主要应用 检测方法 ACT Chromogenic 描述 产品目录编号 ECM504 品牌系列 Chemicon®
商名 - Chemicon
- QCM
描述 QCM Chemotaxis Cell Migration Assay, 24-well (3µm), colorimetric 概述 Also available: Cell Comb™ Scratch Assay! Get biochemical data from a scratch assay!
Introduction
Cell migration is a fundamental function of normal cellular processes, including embryonic development, angiogenesis, wound healing, immune response, and inflammation (1, 2). One such process, leukocyte extravasation, is crucial for appropriate and effective immune response. Neutrophils normally exist in a resting state as they circulate though the body. However, upon interaction with small molecules known as chemoattractants, they rapidly respond with endothelial adhesion followed by emigration from the vasculature and chemotaxis to the site of inflammation. These chemoattractant receptors activate heterotrimeric GTP-binding proteins (G proteins) that initiate numerous elaborate signal transduction cascades, culminating in neutrophil migration and activation. Once at the site of inflammation, neutrophils respond with phagocytosis, superoxide generation, and the release of degradative enzymes.
Microporous membrane inserts are widely used for cell migration and invasion assays. The most widely accepted of which is the Boyden Chamber assay. The Boyden Chamber system uses a hollow plastic chamber, sealed at one end with a porous membrane. This chamber is suspended over a larger well which may contain medium and/or chemoattractants. Cells are placed inside the Chamber and allowed to migrate through the pores, to the other side of the membrane. Migratory cells are then stained and counted.
Most migration assays utilize an 8 µm pore size, as this is appropriate for most cell types, e.g. epithelial and fibroblast cells. The Millipore 3 µm QCM™ Chemotaxis Assay 24-well- colorimetric utilizes a 3 µm pore size, which is appropriate for leukocyte migration. Cells migrated toward chemoattractants were measured by WST-1 (cell viability stain), and quantitated by spectrophotometers. The system may be adapted to study different types of cell migration, including haptotaxis, random migration, and chemokinesis.
The Millipore QCM™ 3 µm Chemotaxis Assay 24-well-colorimetric provides a quick and efficient system for quantitative determination of various factors on cell migration, including screening of pharmacological agents, evaluation of integrins or other adhesion receptors responsible for cell migration, or analysis of gene function in transfected cells.
In addition, Chemicon continues to provide numerous migration, invasion, and adhesion products including:
• QCM™ 8µm 24-well Chemotaxis Cell Migration Assays (ECM508, 509)
• QCM™ 5µm 24-well Chemotaxis Cell Migration Assay (ECM507)
• QCM™ 8µm 96-well Chemotaxis Cell Migration Assay (ECM510)
• QCM™ 5µm 96-well Chemotaxis Cell Migration Assay (ECM512)
• QCM™ 3µm 96-well Chemotaxis Cell Migration Assay (ECM515)
• QCM™ 96-well ECMatrix™ Cell Invasion Assay (ECM555)
• QCM™ 96-well Collagen-based Cell Invasion Assay (ECM556)产品信息 部件 - Forceps: (Part No. 10203) One each.
- Sterile 3 µm 24-well Cell Migration Plate Assembly: (Part No. 2005709) Two 24-well plates with 12 inserts per plate (24 inserts total/kit).
- WST-1 Reagent: (Part No. CS201370) One vial.
- Electro Coupling Solution: (Part No. CS201382) 5 ml.
检测方法 Chromogenic 应用 应用 This 3 µm QCM Chemotaxis Assay 24-well-colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle. 主要应用 - Activity Assay
应用说明 The Millipore 3 µm QCM™ Chemotaxis Assay 24-well-colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle. The 3 µm pore size of this assay's Boyden chambers is appropriate for studying leukocyte migration. The quantitative nature of this assay is useful for screening of pharmacological agents. Each kit provides sufficient materials for the evaluation of 24 samples.
The Millipore 3 µm QCM™ Chemotaxis Assay 24-well-colorimetric is intended for research use only; not for diagnostic applications.生物信息 品种反应性 All 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 The two Sterile 3 µm 24-well Cell migration plate assembly and forceps are stored at 2°-8°C. WST-1 reagent and Electro Coupling Solution are stored at -20°C. 包装信息 数量 1 plate 包装 24 wells
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