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品种反应性 主要应用 宿主 格式 抗体类型 R, M IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB9872 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-TrkB Antibody Alternate Names - Tyrosine kinase receptor B
背景信息 The mammalian Trk family of receptor tyrosine kinases consists of three principle transmembrane proteins. The receptors can be activated by one or more of four neurotrophins - NGF, BDNF, NT3 and NT4/5. The TrkB family of transmembrane proteins serves as receptors for BDNF and NT-4/5 and these neurotrophins regulate biological responses such as cell survivial, proliferation and differentiation in normal and neoplastic neuronal cells. 产品信息 格式 Serum 演示 Lyophilized. Reconstitute with 50 μL of sterile distilled water. Centrifuge to remove any insoluble material. Contains no preservative. 应用 应用 Anti-TrkB Antibody is an antibody against TrkB for use in IH. 主要应用 - Immunohistochemistry
应用说明 Immunohistochemistry: 1:3,000. The antibody has been used successfully on rat dorsal root ganglion and trigeminal ganglia in the rat spinal cord (cryostat free-floating sections). Suggested fixative is 4% paraformaldehyde.
Not suitable for Western blot.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Extracellular domain of the recombinant mouse receptor expressed in mammalian cells. 宿主 Rabbit 特异性 trkB [BDNF/NT-4/5 growth factor receptor; tyrosine kinase receptor B]. The immunogen sequence was chosen to avoid cross reactivity with trkA or trkC. 品种反应性 RatMouse Species Reactivity Note Other species have not yet been tested. It is expected to react with a wide range of mammalian species, based on the high sequence similarity. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006180.3
- NM_001007097.1
- NM_001018064.1
- NM_001018065.1
- NM_001018066.1
Entrez基因汇总 This gene encodes a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. Signalling through this kinase leads to cell differentiation. Mutations in this gene have been associated with obesity and mood disorders. Alternate transcriptional splice variants encoding different isoforms have been found for this gene. 基因符号 - TRKB
- GP145-TrkB
- EC 2.7.10.1
- NTRK2
- Trk-B
纯化方法 Serum UniProt编号 - Q16620
UniProt汇总 FUNCTION: SwissProt: Q16620 # Receptor for brain-derived neurotrophic factor (BDNF), neurotrophin-3 and neurotrophin-4/5 but not nerve growth factor (NGF). Involved in the development and/or maintenance of the nervous system. This is a tyrosine-protein kinase receptor. Known substrates for the TRK receptors are SHC1, PI-3 kinase, and PLC- gamma-1.
SIZE: 822 amino acids; 91999 Da
SUBUNIT: Exists in a dynamic equilibrium between monomeric (low affinity) and dimeric (high affinity) structures. Binds SH2B2. Interacts with SQSTM1 (By similarity).
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Isoform TrkB is widely expressed, mainly in the nervous tissue. In the CNS, expression is observed in the cerebral cortex, hippocampus, thalamus, choroid plexus, granular layer of the cerebellum, brain stem, and spinal cord. In the peripheral nervous system, it is expressed in many cranial ganglia, the ophtalmic nerve, the vestibular system, multiple facial structures, the submaxillary glands, and dorsal root ganglia. Isoform TrkB-T1 is expressed in multiple tissues, mainly in brain, pancreas, kidney and heart. Isoform TrkB-T-Shc is predominantly expressed in brain.
PTM: Ligand-mediated auto-phosphorylation.
SIMILARITY: SwissProt: Q16620 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 Ig-like C2-type (immunoglobulin-like) domains. & Contains 2 LRR (leucine-rich) repeats. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material frozen at -20°C to -70°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1, ACS or better grade) can be added for additional stability. 包装信息 数量 50 µL
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