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品种反应性 主要应用 宿主 格式 抗体类型 R IHC, WB M Purified Monoclonal Antibody 描述 产品目录编号 MABN20 描述 Anti-HCN1 Antibody, clone N70/28 Alternate Names - Brain cyclic nucleotide-gated channel 1
- hyperpolarization activated cyclic nucleotide-gated potassium channel 1
- brain cyclic nucleotide gated channel 1
背景信息 HCN1 is a member of a family of pacemaker channels activated by hyperpolarization and regulated by cyclic nucleotides. HCN1 and HCN2 proteins play an important role for motor learning and synapse formation through integration of cerebellar Purkinje cells. The two proteins also shape autonomous activity of single neurons and the periodicity of network oscillations. HCN1 expression is particularly high in the cerebral cortex, hippocampus, cerebellum, and facial motor nucleus. Hyperpolarization of activated cation channels of the HCN gene family such as HCN1, contributes to spontaneous rhythmic activity in both heart and brain. HCN channels pass both Na+ and K+ with relative permeability ratio of ~0.15 to 0.25. The primary sequence of HCN pore region is clearly related to that of highly selective K+ channels. In contrast to nonselective CNG or Na+-channels, both K+ and HCN channels contain a GYG motif in the pore. 产品信息 格式 Purified 控制 - Rat brain lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Detect HCN1 using this Anti-HCN1 Antibody, clone N70/28 validated for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunohistochemistry Analysis: 1:300 dilution from a previous lot detected HCN1 in rat cerebellum and rat brain/inferior colliculus tissue. 生物信息 免疫原品种 Recombinant protein corresponding to rat HCN1 at the cytoplasmic domain. 表位 Cytoplasmic domain 克隆 N70/28 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes HCN1 at the cytoplasmic domain. 同种型 IgG1κ 品种反应性 Rat Species Reactivity Note Demonstrated to react with rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_445827
Entrez基因汇总 May generate neuronal pacemaker activity [RGD]. 基因符号 - HAC-2
- HCN1
- BCNG1
- BCNG-1
纯化方法 Protein G UniProt编号 - Q9JKB0
UniProt汇总 FUNCTION: Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP (By similarity). May mediate responses to sour stimuli.
SUBUNIT: The potassium channel is probably composed of a homo- or heterotetrameric complex of pore-forming subunits. Heteromultimer with HCN2 (By similarity). Interacts with KCNE2 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
TISSUE SPECIFICITY: Highly expressed in cerebral cortex, cerebellum, throughout the hippocampus, in medial habenula, anterior dorsal nucleus in the thalamus, tenia tecta, several nuclei of the general motor system and in optic nerve layer. Detected in a subset of elongated cells in taste buds.
DOMAIN: The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
SIMILARITY: Belongs to the potassium channel HCN family.
SIMILARITY: Contains 1 cyclic nucleotide-binding domain.产品使用声明 质量保证 Evaluated by Western Blot in rat brain lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected HCN1 on 10 µg of rat brain lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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