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Replacement Information Replacement Information 07-1221 is a recommended replacement for AB4088 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, B, H, M WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1221 Replaces AB4088 描述 Anti-PP2A-B’ (B56) Antibody Alternate Names - protein phosphatase 2A, regulatory subunit B' (PR 53)
- PP2A phosphatase activator
- PP2A, subunit B'
- PP2A, subunit B', PR53 isoform
- Phosphotyrosyl phosphatase activator
- protein phosphatase 2A activator, regulatory subunit 4
- protein phosphatase 2A, regulatory subunit B'
- protein phosphatase 2A activator
- regulatory subunit 4
- protein phosphatase 2A
- regulatory subunit B'
背景信息 PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C). The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits. There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 . The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma . The B' (or B56) family consists of at least seven splice variants derived from five genes. RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene. Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals. PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid. It is also thought to be the principal downmodulator of Ser/Thr kinase cascades. 产品信息 格式 Purified 控制 - HeLa Cell Lysate
演示 Purified rabbit polyclonal IgG in PBS with 0.08% sodium azide. 应用 应用 Detect PP2A-B’ (B56) using this Anti-PP2A-B’ (B56) Antibody validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 The immunogen used for the purified peptide conjugated to KLH corresponds to the sequence NH2-Val-Ser-Ser-Pro-His-Phe-Gln-Val-Ala-Glu- Arg-Ala-Leu-Tyr-COOH. 表位 Internal 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody is specific to PP2A-B’ subunits. 同种型 IgG 品种反应性 RatBovineHumanMouse Species Reactivity Note Bovine, Human, Mouse, Rat. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_006243
Entrez基因汇总 The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an epsilon isoform of the regulatory subunit B56 subfamily. 基因符号 - PTPA
- MGC2184
- OTTHUMP00000022333
- PP2A
- PR53
纯化方法 Purified UniProt编号 - Q15257
UniProt汇总 FUNCTION: Reversibly stimulates the variable phosphotyrosyl phosphatase activity of PP2A core heterodimer in presence of ATP and Mg2+ (in vitro).
SUBUNIT STRUCTURE:Associates with PP2A heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A).
TISUE SPECIFICITY: Widely expressed.
SEQUENCE SIMILARITY: Belongs to the PTPA-type PPIase family.产品使用声明 质量保证 Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500-1:1,500 dilution of this antibody detected PP2A-B' (B56) in 10 μg of multiple cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µg
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