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检测方法 Colorimetric 描述 概述 The InnoZyme™ Myeloperoxidase Activity Kit is a specific and sensitive assay designed to measure myeloperoxidase activity and to screen enzyme inhibitors. The assay uses a polyclonal antibody specific for human MPO immobilized on a 96-well plate to specifically capture the enzyme. Activity of captured myeloperoxidase is measured using a detection reagent that includes TMB and hydrogen peroxide. Following color development, the reaction is stopped with sulfuric acid and the absorbance of the oxidized TMB is detected at 450 nm. 产品目录编号 CBA024 品牌系列 Calbiochem®
同义词 InnoZyme™ MPO Activity Kit 应用数据
参考 参考 Weil, S.C., et al. 1988. Science 240, 790.
Winterbourn, C.C., et al. 2000. Curr. Opin. Hematol. 7, 53.
Brennan, M.L., et al. 2003. New Engl. J Med. 349, 1595.
Zheng, L., et al. 2004. J. Biol. Chem. in press
Vita, J.A., et al. 2004. Circulation, 110, 1134.
产品信息 检测方法 Colorimetric 形式 96 Tests 格式 96-well plate 试剂盒包含 Anti-MPO Coated 96-Well Plate, MPO Standard, TMB, Hydrogen Peroxide, Assay Buffer, Sample Buffer, Stop Solution, Plate Sealers, and a user protocol. 正控制 Human myeloperoxidase 生物信息 化验范围 5 -100 ng/ml 化验时间 2 h 样品类型 Cell lysates, CSF, and serum 品种 human 理化信息 灵敏度 330 pg/ml 安全信息 R相 R: 25-35-36/38-43-62
Toxic if swallowed.
Causes severe burns.
Irritating to eyes and skin.
May cause sensitization by skin contact.
Possible risk of impaired fertility.S相 S: 24-26-28.1-3-30-36/37/39-45
Avoid contact with skin.
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
After contact with skin, wash immediately with plenty of water.
Keep in a cool place.
Never add water to this product.
Wear suitable protective clothing, gloves and eye/face protection.
In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).产品使用声明 预定用途 The Calbiochem® InnoZyme™ Myeloperoxidase Activity Kit is designed to measure human myeloperoxidase activity in cell lysates and biological samples and to screen enzyme inhibitors. 储存和货运信息 货运代码 Dry Ice Only
毒性 Multiple Toxicity Values, refer to MSDS
有害材料注意事项: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
储存 -20°C
存储条件 Upon arrival, store the unopened kit at -20°C. All kit components, once opened, can be stored for up to 3 months under the following conditions:
Store at 4°C: TMB, Anti-MPO Coated 96-Well Plate, Assay Buffer, Stop Solution, and Sample Buffer
Store at -20°C: Myeloperoxidase Standard, and Hydrogen Peroxide
Note: Following initial use the Myeloperoxidase Standard and the Hydrogen Peroxide should be dispensed into aliquots and stored at -20°C. Avoid freeze/thaw cycles.避光 Protect from light
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
补充信息 试剂盒包含 Anti-MPO Coated 96-Well Plate, MPO Standard, TMB, Hydrogen Peroxide, Assay Buffer, Sample Buffer, Stop Solution, Plate Sealers, and a user protocol.
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