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merck millipore,默克密理博,FCMAB250A4,Milli-Mark Anti-Bcl-2 Antibody, Alexa Fluor488, clone AW604
产品名称：Milli-Mark Anti-Bcl-2 Antibody, Alexa Fluor488, clone AW604
产品型号：FCMAB250A4
This Milli-Mark Bcl-2 antibody is validated for use in Flow for the detection of the Milli-Mark Bcl-2 protein.
merck millipore,默克密理博,FCMAB250A4,Milli-Mark Anti-Bcl-2 Antibody, Alexa Fluor488, clone AW604
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H FC M AlexaFluor®488 Monoclonal Antibody 描述 产品目录编号 FCMAB250A4 商名 - Milli-Mark
描述 Milli-Mark Anti-Bcl-2 Antibody, Alexa Fluor®488, clone AW604 背景信息 Bcl-2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Bcl-2 also regulates cell death by controlling the mitochondrial membrane permeability and appears to function in a feedback loop system with caspases. It appears to Inhibit caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Further understanding in how Bcl-2 functions and interacts with other proteins to promote or inhibit apoptosis may lead to new treatments for cancer, neurological disorders and autoimmune diseases. 产品信息 格式 AlexaFluor®488 控制 - Jurkat Cells
演示 Purified mouse monoclonal IgG2a conjugated to Alexa Fluor® 488 in PBS with 0.1% sodium azide and 15 mg/mL BSA. 应用 应用 This Milli-Mark Bcl-2 antibody is validated for use in Flow for the detection of the Milli-Mark Bcl-2 protein. 主要应用 - Flow Cytometry
生物信息 免疫原品种 KLH-conjugated, synthetic peptide corresponding to human Bcl-2 克隆 AW604 宿主 Mouse 特异性 Antibody recognizes Bcl-2. 同种型 IgG2a 品种反应性 Human Species Reactivity Note Tested and passed on Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_000624
Entrez基因汇总 This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants, produced by alternate splicing, differ in their C-terminal ends. 基因符号 - Bcl-2
- BCL2
纯化方法 Protein G Purified UniProt编号 - P10415
UniProt汇总 FUNCTION: SwissProt: P10415 # Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
SIZE: 239 amino acids; 26266 Da
SUBUNIT: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity (By similarity). Also interacts with APAF1, RAF-1, TP53BP2, BBC3, BCL2L1, MRPL41 and BNIPL. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets.
SUBCELLULAR LOCATION: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein.
TISSUE SPECIFICITY: Expressed in a variety of tissues.
DOMAIN: SwissProt: P10415 The BH4 motif is required for anti-apoptotic activity and for interaction with RAF-1.
PTM: Phosphorylation/dephosphorylation on Ser-70 regulates anti- apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Dephosphorylated by protein phosphatase 2A (PP2A) (By similarity). & Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
DISEASE: SwissProt: P10415 # A chromosomal aberration involving BCL2 may be a cause of follicular lymphoma (FL) [MIM:151430]; also known as type II chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
SIMILARITY: Belongs to the Bcl-2 family产品使用声明 质量保证 Evaulated by flow cytometery using Jurkat cells. 使用声明 - Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
储存和货运信息 存储条件 Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 tests
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