merck millipore,默克密理博,07-024,Anti-gp91-phox Antibody

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merck millipore,默克密理博,07-024,Anti-gp91-phox Antibody
产品名称：Anti-gp91-phox Antibody
产品型号：07-024
Anti-gp91-phox Antibody is an antibody against gp91-phox for use in WB.

merck millipore,默克密理博,07-024,Anti-gp91-phox Antibody

产品介绍

merck millipore,默克密理博,07-024,Anti-gp91-phox Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-024
品牌系列	Upstate
商名	Upstate
描述	Anti-gp91-phox Antibody
Alternate Names	p22 phagocyte B-cytochrome Cytochrome b(558) subunit beta Cytochrome b558 subunit beta Heme-binding membrane glycoprotein gp91phox NADPH oxidase Neutrophil cytochrome b 91 kDa polypeptide Superoxide-generating NADPH oxidase heavy chain subunit chronic granulomatous disease cytochrome b-245 beta polypeptide cytochrome b-245, beta polypeptide beta polypeptide
背景信息	Cytochrome b-245, beta polypeptide (chronic granulomatous disease), also known as CYBB and P91-PHOX, is a human gene encoding a glycoprotein. Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles.

产品信息
格式	Purified
控制	Lung tissue, human neutrophil membrane, HL60 membrane and RIPA cell lysate.
演示	Purified rabbit polyclonal IgG in buffer containing 0.07 M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide and 30% glycerol. Liquid at -20°C.

应用
应用	Anti-gp91-phox Antibody is an antibody against gp91-phox for use in WB.
主要应用	Western Blotting
应用说明	Immunoprecipitation: Not recommended.

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to amino acids 548-560 of human gp91-phox (KQSISNSESGPRG). The immunizing sequence is identical in mouse, and it shares 11/13 sequence homology in bovine and 10/13 in porcine.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Detects gp91-phox, Mr 75 kDa. Depending on the type of lysate, the amount loaded, the gel and the blotting conditions, gp91-phox may run as a sharp band, Mr 75 kDa, or appear as a smear between 75-91 kDa.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human. Predicted to cross react with mouse.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_000397
Entrez基因汇总	Cytochrome b (-245) is composed of cytochrome b alpha (CYBA) and beta (CYBB) chain. It has been proposed as a primary component of the microbicidal oxidase system of phagocytes. CYBB deficiency is one of five described biochemical defects associated with chronic granulomatous disease (CGD). In this disorder, there is decreased activity of phagocyte NADPH oxidase; neutrophils are able to phagocytize bacteria but cannot kill them in the phagocytic vacuoles. The cause of the killing defect is an inability to increase the cell's respiration and consequent failure to deliver activated oxygen into the phagocytic vacuole.
基因符号	p91-PHOX CGD CGD91-phox EC 1.-.-.- GP91-1 GP91-PHOX GP91PHOX NOX2 OTTHUMP00000031915 gp91-1 gp91-phox
纯化方法	Protein A purfied
UniProt编号	P04839
UniProt汇总	FUNCTION: SwissProt: P04839 # Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. It is the terminal component of a respiratory chain that transfers single electrons from cytoplasmic NADPH across the plasma membrane to molecular oxygen on the exterior. Also functions as a voltage-gated proton channel that mediates the H(+) currents of resting phagocytes. It participates in the regulation of cellular pH and is blocked by zinc. COFACTOR: FAD (Probable). SIZE: 570 amino acids; 65336 Da SUBUNIT: Composed of a heavy chain (beta) and a light chain (alpha). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. PTM: Glycosylated. DISEASE: SwissProt: P04839 # Defects in CYBB are a cause of X-linked chronic granulomatous disease (X-CGD) [MIM:306400]. X-CGD is characterized by the failure of activated phagocytes to generate superoxide. Patients suffer from life-threatening bacterial/fungal infections. Patients with CYBB dysfunction are generally classified into three groups, namely X91(0) (no expression of the protein); X91(-) (reduced expression) and X91(+) (normal expression). SIMILARITY: SwissProt: P04839 ## Contains 1 ferric oxidoreductase domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on human neutrophil membranes. Western Blot Analysis: 1-4 µg/mL of this lot detected gp91-phox in a HL60 membrane and RIPA cell lysate. A previous lot detected gp91-phox in human neutrophil membranes.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.