merck millipore,默克密理愽
  • merck millipore,默克密理博,SCR047,Pancreatic Cell DTZ Detection Assay

  • merck millipore,默克密理博,SCR047,Pancreatic Cell DTZ Detection Assay

    产品名称：Pancreatic Cell DTZ Detection Assay
    产品型号：SCR047
    The Pancreatic Cell DTZ Detection Assay provides researcher a simple & quick method to identify insulin-producing beta cells from a mixed cell culture preparation or from pancreatic tissues.

    merck millipore,默克密理博,SCR047,Pancreatic Cell DTZ Detection Assay

  • 产品介绍
  • merck millipore,默克密理博,SCR047,Pancreatic Cell DTZ Detection Assay

    重要规格表

    检测方法
    Chromogenic
    描述
    产品目录编号 SCR047
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Pancreatic Cell DTZ Detection Assay
    概述 The Pancreatic Cell DTZ Detection Assay provides researcher a simple and quick method to identify insulin-producing beta cells from a mixed cell culture preparation or from pancreatic tissues The kit contains pre-mixed Dithizone Stain, Rinsing Solution and filters for fast preparation and optimized results. Millipore's Pancreatic Cell DTZ Detection Asssay (SCR047) takes advantage of the high zinc content of pancreatic beta cells and the known action of dithizone, a zinc-chelating agent, to provide a quick and simple enzymatic reaction that allows researcher to reliably identify insulin-producing beta cells from a mixed cell culture preparation or from pancreatic tissues. Along with selectively staining live pancreatic beta-islet cells crimson red, the enzymatic reaction has the advantage of being completely reversible. Kit components include staining and rinse solutions along with filters required for the live staining reactions. Sufficient reagents are supplied to provide for 10 enzymatic reactions at 10 mL volume.
    背景信息 The islets of Langerhans are groups of specialized cells that are dispersed throughout the pancreas. They produce a number of hormones including glucagon, insulin, Pancreatic polypeptide and somatostatin. Insulin is the most critical of these, as it helps regulate glucose metabolism. Insulin is synthesized from a precursor molecule, proinsulin, in the beta cells of the islets of Langerhans. The conversion of proinsulin into the active hormone, insulin, is accomplished by proteolytic enzymes known as prohormone convertases (PC1 and PC2) as well as the exoprotease carboxypeptidase E. These proteolytic enzymes remove the center portion of the molecule, or C-peptide, and the remaining peptide chains (A and B) are cross-linked by disulfide bonds. The crosslinked peptides are the insulin monomer. As the concentration of the protein increases, the monomers assemble into dimers and in the presence of zinc, into hexameric crystals characterized by two Zn2+ atoms per hexameric unit. It is this 2-Zn insulin hexamer that represents the active conformation of the secreted hormone. Given its prominent role in the synthesis, storage and secretion of insulin, it is not surprising that zinc is highly expressed in pancreatic beta cells.
    产品信息
    部件
    • 12 Plastic Syringes, 20 mL w/ Luer slip (Part No: XX1102012)
    • 1 mL of 100X DTZ Stain Solution (Part No: 2006182)
    • 2 - 150 mL of 1X DTZ Rinse Solution (Part No: 2006183)
    • 10 Millex GP 0.22 uM filters (Part No: SLGP033RB)
    检测方法 Chromogenic
    应用
    应用 The Pancreatic Cell DTZ Detection Assay provides researcher a simple & quick method to identify insulin-producing beta cells from a mixed cell culture preparation or from pancreatic tissues.
    生物信息
    品种反应性 Mammals
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Store DTZ Stain at -20C. Store all other reagents at room temperature.
    包装信息
    数量 1 kit

    merck millipore,默克密理博,SCR047,Pancreatic Cell DTZ Detection Assay

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