merck millipore,默克密理博,06-933,Anti-acetyl-Lysine Antibody

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merck millipore,默克密理博,06-933,Anti-acetyl-Lysine Antibody
产品名称：Anti-acetyl-Lysine Antibody
产品型号：06-933
Anti-acetyl-Lysine Antibody is a high quality Rabbit Polyclonal Antibody for the detection of acetyl-Lysine & has been validated in WB & IP.

merck millipore,默克密理博,06-933,Anti-acetyl-Lysine Antibody

产品介绍

merck millipore,默克密理博,06-933,Anti-acetyl-Lysine Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Vrt	WB	Rb	Serum	Polyclonal Antibody

描述
产品目录编号	06-933
品牌系列	Upstate
商名	Upstate
描述	Anti-acetyl-Lysine Antibody
背景信息	In the nucleus, DNA is tightly packed into nucleosomes generating an environment which is highly repressive towards DNA processes such as transcription. Acetylation of lysine residues within proteins has emerged as an important mechanism used by cells to overcome this repression. The acetylation of non-histone proteins such as transcription factors, as well as histones appears to be involved in this process. Acetylation may result in structural transitions as well as specific signaling within discrete chromatin domains. The role of acetylation in intracellular signaling has been inferred from the binding of acetylated peptides by the conserved bromodomain. Furthermore, recent findings suggest that bromodomain/acetylated-lysine recognition can serve as a regulatory mechanism in protein-protein interactions in numerous cellular processes such as chromatin remodeling and transcriptional activation.

产品信息
格式	Serum
控制	TSA-treated HeLa and NIH/3T3 cells, osteosarcoma tissue.
演示	Rabbit IgG serum containing 0.05% sodium azide and 30% glycerol.

应用
应用	Anti-acetyl-Lysine Antibody is a high quality Rabbit Polyclonal Antibody for the detection of acetyl-Lysine & has been validated in WB & IP.
主要应用	Western Blotting
应用说明	Immunoprecipitation: 5 μL of a previous lot immunoprecipitated in vitro acetylated recombinant p300. Western Blot Analysis: A 1:1000 dilution of the previous lot of the antibody detected: • Acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5 μM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B). • GST-p53 (100 ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C).

生物信息
免疫原品种	A mixture of chemically acetylated antigens.
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Acetyl-lysine containing proteins including histones, from sodium butyrate or Trichostatin A treated cells, p53 acetylated in vitro by p300 or PCAF, and chemically acetylated BSA.
同种型	IgG
品种反应性	Vertebrates
Species Reactivity Note	Broad species reactivity expected.
抗体类型	Polyclonal Antibody
修改	Acetylation
纯化方法	Antiserum

产品使用声明
质量保证	Evaluated by western blot on acid-extracted proteins from sodium butyrate treated HeLa cells, chemically acetylated BSA, acetylated histones and to a lesser degree, other proteins in trichostatin A treated Cos-1 cell lysate. Western Blot Analysis: 1:1000-1:2000 dilution of this antibody detected: • Acetylated histones in acid-extracted proteins from sodium butyrate (5 mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A). • Chemically acetylated BSA (10 ng) in a dot blot assay.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Evaluated by western blot on acid-extracted proteins from sodium butyrate treated HeLa cells, chemically acetylated BSA, acetylated histones and to a lesser degree, other proteins in trichostatin A treated Cos-1 cell lysate.

Western Blot Analysis: 1:1000-1:2000 dilution of this antibody detected:
• Acetylated histones in acid-extracted proteins from sodium butyrate (5 mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A).
• Chemically acetylated BSA (10 ng) in a dot blot assay.

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.