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merck millipore,默克密理博,16-305,Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor 488
产品名称：Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor 488
产品型号：16-305
Detect phospho-Akt1/PKBα (Ser473) using this Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor 488 validated for use in FC, IC, IF & WB.
merck millipore,默克密理博,16-305,Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor 488
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, Ca, H, M WB, FC, ICC M AlexaFluor®488 Monoclonal Antibody 描述 产品目录编号 16-305 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor® 488 Alternate Names - PKB, PKD-alpha, AKT1, Protein Kinase B
- PKB
- PKD-alpha
- AKT1
产品信息 格式 AlexaFluor®488 应用 应用 Detect phospho-Akt1/PKBα (Ser473) using this Anti-phospho-Akt1/PKBα (Ser473) Antibody, clone 11E6, Alexa Fluor 488 validated for use in FC, IC, IF & WB. 主要应用 - Western Blotting
- Flow Cytometry
- Immunocytochemistry
生物信息 免疫原品种 KLH conjugated synthetic peptide containing a pSer that corresponds to amino acid residues around phosphoserine 473. 克隆 11E6 宿主 Mouse 特异性 Recognizes phosphorylatedAkt1/PKBα at Ser473. This lot of antibody does not cross-react with Akt1/PKBα dephosphorylated at Ser473. 同种型 IgG1 品种反应性 RatCanineHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005163.2
- NM_001014431.1
- NM_001014432.1
Entrez基因汇总 The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. 基因符号 - AKT
- AKT1
- RAC
- PRKBA
- MGC99656
- RAC-ALPHA
- RAC-PK-alpha
- C-AKT
- PKB
修改 - Phosphorylation
纯化方法 Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography UniProt编号 - P31749
UniProt汇总 FUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
SIZE: 480 amino acids; 55686 Da
SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
TISSUE SPECIFICITY: In all human cell types so far analyzed.
DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by immunocytochemistry and flow cytometry. 使用声明 - Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 1 year at 4°C from date of shipment 包装信息 数量 50 µg
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