merck millipore,默克密理博,MABE955,Anti-phospho RNA Pol II (Ser7), clone 3D4A12 Antibody

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Merck Millipore 默克密理愽

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M, Ca, Mk	ELISA, WB, ChIP, ICC	R	Purified	Monoclonal Antibody

描述
产品目录编号	MABE955
描述	Anti-phospho RNA Pol II (Ser7), clone 3D4A12 Antibody
Alternate Names	RNAPIIS7p DNA-directed RNA polymerase II subunit RPB1 RNA polymerase II subunit B1 DNA-directed RNA polymerase II subunit A DNA-directed RNA polymerase III largest subunit RNA-directed RNA polymerase II subunit RPB1 RNAPII RNA PolII
背景信息	As the study of the RNA polymerase II (RNAPII) transcription complex expands, many researchers are interested in using immunoblots to detect RNAPII in various protein preparations and column fractions. HeLa cell RNAPII contains 10 subunits, the largest possessing a heptapeptide repeat (PTSPSYS) in the C-terminal domain (CTD). This large subunit cycles, during the course of transcription, through nonphosphorylated (RNAPIIA) and hyperphosphorylated (RNAPIIO) forms. RNAPIIA associates with the basal transcription complex and becomes phosphorylated to the RNAPIIO form before the synthesis of the first phosphodiester bond. The timing of these events suggests that this phosphorylation event may function as a regulatory point, and there is considerable interest in identifying components of the transcription complex with CTD kinase activity.

产品信息
格式	Purified
演示	Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

应用
应用	This Anti-phospho RNA Pol II (Ser7) antibody, clone 3D4A12 is validated for use in western blotting, ChIP-seq, ChIP, ICC & ELISA for the detection of phospho RNA Pol II (Ser7).
主要应用	ELISA Western Blotting Chromatin Immunoprecipitation (ChIP) Immunocytochemistry
应用说明	Chromatin Immunoprecipitation-Sequencing Analysis: A representative lot from an independent laboratory immunoprecipitated phospho RNA Pol II (Ser7) from HeLa cell lysate (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.). Chromatin Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated phospho RNA Pol II (Ser7) from HeLa cell lysate (Maehara, K., et al. (2013). Nucleic Acids Res. 41(1):54-62.). Western Blotting Analysis: 1 µg/mL from a representative lot detected phospho RNA Pol II (Ser7) in HeLa (lane 1), COS1, L929, NRK, MDCK, and DM4 whole cell extracts (Prof. Taro Tachibana, Cell Engineering Corporation.). Immunocytochemistry Analysis: A 1:500 dilution from representative lot detected phospho RNA Pol II (Ser7) in HeLa cells (Prof. Taro Tachibana, Cell Engineering Corporation.). ELISA Analysis: A representative lot detected phospho RNA Pol II (Ser7) in a panel of unmodified and modified RNA Pol II peptides (Prof. Taro Tachibana, Cell Engineering Corporation.).

生物信息
免疫原品种	KLH-conjugated linear peptide corresponding to human phospho RNA Pol II (Ser7).
克隆	3D4A12
浓缩	1 mg/mL
宿主	Rat
同种型	IgG2bκ
品种反应性	RatHumanMouseCanineMonkey
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_000928
基因符号	POLR2A
纯化方法	Protein G purified
UniProt编号	P24928

产品使用声明
质量保证	Evaluated by Western Blotting in HeLa cell lysate. Western Blotting Analysis: 4 µg/mL of this antibody detected phospho RNA Pol II (Ser7) in 10 µg of HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt.

包装信息
数量	200 µg

merck millipore,默克密理博,MABE955,Anti-phospho RNA Pol II (Ser7), clone 3D4A12 Antibody

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