merck millipore,默克密理博,06-418,Anti-NFκB p65 Antibody, CT

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merck millipore,默克密理博,06-418,Anti-NFκB p65 Antibody, CT
产品名称：Anti-NFκB p65 Antibody, CT
产品型号：06-418
Anti-NFκB p65 Antibody, CT is a Rabbit Polyclonal Antibody for detection of NFκB p65 also known as Nuclear factor NF-kappa-B p65 subunit & has been validated in EMSA, IP & WB.

merck millipore,默克密理博,06-418,Anti-NFκB p65 Antibody, CT

产品介绍

merck millipore,默克密理博,06-418,Anti-NFκB p65 Antibody, CT

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H, R	WB, EMSA	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	06-418
Replaces	04-1008; 04-235
品牌系列	Upstate
商名	Upstate
描述	Anti-NFκB p65 Antibody, CT
Alternate Names	v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 (avian) Nuclear factor NF-kappa-B p65 subunit v v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear<br />factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)) v-rel reticuloendotheliosis viral oncogene homolog A (avian) v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 nuclear factor of kappa light polypeptide gene enhancer in B-cells 3 p65
背景信息	The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.

产品信息
格式	Purified
控制	TNFα treated HeLa cells, PMA, A431 cells or calcium ionophore-treated Jurkat cells Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
演示	Purified rabbit polyclonal IgG in 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20°C.

应用
应用	Anti-NFκB p65 Antibody, CT is a Rabbit Polyclonal Antibody for detection of NFκB p65 also known as Nuclear factor NF-kappa-B p65 subunit & has been validated in EMSA, IP & WB.
主要应用	Western Blotting Electrophoretic Mobility Shift Assay
应用说明	Immunoprecipitation: 4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.

生物信息
免疫原品种	KLH-conjugated, synthetic peptide corresponding to the 13 C-terminal amino acids (C-DMDFSALLSQISS) of the p65 subunit of human NFκB containing an N-terminal cysteine for conjugation. The immunizing sequence is identical in mouse.
表位	C-terminus
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	NFκB p65, Mr 65 kDa
同种型	IgG
品种反应性	MouseHumanRat
Species Reactivity Note	Human, mouse and rat
抗体类型	Polyclonal Antibody
Entrez基因编号	NP_068810
Entrez基因汇总	NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T; R is an A or G purine; and Y is a C or T pyrimidine).[supplied by OMIM]
基因符号	NFKB3 RELA MGC131774
纯化方法	Protein A chromatography
UniProt编号	Q04206
UniProt汇总	FUNCTION: SwissProt: Q04206 # p65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF- kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. SIZE: 551 amino acids; 60219 Da SUBUNIT: Active NF-kappa-B is a heterodimer of an about 50 kDa DNA-binding subunit and the weak DNA-binding subunit p65. Two heterodimers might form a labile tetramer. May interact with ETHE1. Binds AES and TLE1. Interacts with TP53BP2. Binds to and is phosphorylated by the activated form of either RPS6KA4 or RPS6KA5. Interacts with ING4 and this interaction may be indirect. Interacts with CARM1, USP48 and UNC5CL. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B). PTM: Ubiquitinated, leading to its proteosomal degradation. Degradation is required for termination of NF-kappa-B response. SIMILARITY: SwissProt: Q04206 ## Contains 1 RHD (Rel-like) domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on RIPA lysates from A431 cells. Western Blot Analysis: 0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.

Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.

包装信息
数量	100 µg

merck millipore,默克密理博,06-418,Anti-NFκB p65 Antibody, CT

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