merck millipore,默克密理博,04-401,Anti-PI3 Kinase Antibody, p110δ rabbit monoclonal

搜索"Merck Millipore 密理愽"产品

Merck Millipore 默克密理愽

当前位置：首页 > >Merck Millipore > 生命科学研究
生命科学研究

merck millipore,默克密理博,04-401,Anti-PI3 Kinase Antibody, p110δ rabbit monoclonal
产品名称：Anti-PI3 Kinase Antibody, p110δ rabbit monoclonal
产品型号：04-401
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PI3 Kinase Antibody, p110δ Rabbit is an antibod More>>

merck millipore,默克密理博,04-401,Anti-PI3 Kinase Antibody, p110δ rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-401,Anti-PI3 Kinase Antibody, p110δ rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-401
品牌系列	Upstate
商名	Upstate
描述	Anti-PI3 Kinase Antibody, p110δ rabbit monoclonal
Alternate Names	phosphoinositide-3-kinase, catalytic, delta polypeptide PI3-kinase p110 subunit delta PtdIns-3-kinase p110 phosphatidylinositol 3-kinase, catalytic, delta polypeptide phosphoinositide-3-kinase C delta polypeptide phosphoinositide-3-kinase C
背景信息	PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival signaling, and insulin action. PI3 Kinase is a heterodimeric complex composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospho-lipid substrate on the membrane. PI3 Kinase is also activated by Ras, and by the β:γ subunits of heterotrimeric G-proteins. PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.

产品信息
格式	Purified
控制	Jurkat cell lysate
演示	Purified rabbit monoclonal IgG in buffer containing 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-PI3 Kinase Antibody, p110δ Rabbit is an antibody against PI3 Kinase for use in IF, IP & WB.
主要应用	Western Blotting
应用说明	Immunoprecipitation: A 1:40 dilution of a prevoius lot of this antibody immunoprecipitated PI3 Kinase, p110δ using protein G:agarose beads. Immunfluorescence Staining: A 1:250 dilution of a previous lot of this antibody was used for Immunfluorescence staining of paraformaldehyde fixed HeLa cells.

生物信息
免疫原品种	KLH-conjugated synthetic peptide corresponding to human PI3 Kinase, p110δ
宿主	Rabbit
特异性	This antibody recognizes human PI3 Kinase, p110δ.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Proven to react with human. Not believed to cross-react with mouse or rat. Reactivity with other species has not been confirmed.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_005026.2
Entrez基因汇总	Phosphoinositide 3-kinases (PI3Ks) phosphorylate the 3-prime OH position of the inositol ring of inositol lipids. See MIM 602838. The class I PI3Ks display a broad phosphoinositide lipid substrate specificity and include p110-alpha (MIM 171834), p110-beta (MIM 602925), and p110-gamma (MIM 601232). p110-alpha and p110-beta interact with SH2/SH3-domain-containing p85 adaptor proteins (see MIM 171833) and with GTP-bound Ras.[supplied by OMIM]
基因符号	p110D PIK3CD p110d p110delta PI3K
纯化方法	Purified
UniProt编号	O00329
UniProt汇总	SIZE: 1044 amino acids; 119479 Da SUBUNIT: Heterodimer of a p110 (catalytic) and a p85 (regulatory) subunit. Interacts with ERAS (By similarity). TISSUE SPECIFICITY: Expressed predominantly in leukocytes. PTM: Autophosphorylation on Ser-1039 results in the almost complete inactivation of the lipid kinase activity. SIMILARITY: SwissProt: O00329 ## Belongs to the PI3/PI4-kinase family. & Contains 1 PI3K/PI4K domain.

产品使用声明
质量保证	Routinely evaluated by Western Blot on Jurkat cell lysate. Western Blot Analysis: A 1:500 dilution of this lot detected endogenous PI3 Kinase, p110δ in lysate from Jurkat cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.

Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20ºC. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20ºC may cause glycerol-containing solutions to become frozen during storage.