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品种反应性 主要应用 宿主 格式 抗体类型 Chp, H, M, R WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1104 描述 Anti-P/CAF Antibody Alternate Names - Histone acetyltransferase KAT2B
- CREBBP-associated factor
- Histone acetylase PCAF
- K(lysine) acetyltransferase 2B
- P300/CBP-associated factor
背景信息 P/CAF(sometimes called KAT2B) is a transcription cofactor with a histone acetyltransferase (HAT) activity. Consistent with this protein playing a direct role in transcriptional regulation, P/CAF can acetylate core histones H3 and H4, chromatin proteins HMG17 and HMG I(Y), as well as human immunodeficiency virus Tat, transcription factor MyoD, and general transcription factors TFIIE and TFIIF. A role for P/CAF in cell growth and differentiation is suggested by its interaction in vivo and in vitro with p300/CBP, two large nuclear proteins that bind to sequence-specific factors involved in cell growth/differentiation. A role for P/CAF in cell division is also suggested by data showing that this protein competes with E1A for binding sites in p300/CBP to counteract E1A's mitogenic activity.
产品信息 格式 Affinity Purified 控制 - A431 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-P/CAF Antibody (Rabbit Polyclonal Antibody) validated in WB to detect P/CAF also known as CREBBP-associated factor or Histone acetylase PCAF. 主要应用 - Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 1 µg/ml from a representative lot detected P/CAF on 10 µg of A431 cell lysate. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the internal domain of human P/CAF. 表位 Internal 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the internal domain of P/CAF. 品种反应性 ChimpanzeeHumanMouseRat Species Reactivity Note Demonstrated to react with human, mouse, and rat. Predicted to react with chimpanzee and rhesus monkey based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_003875
Entrez基因汇总 CBP and p300 are large nuclear proteins that bind to many sequence-specific factors involved in cell growth and/or differentiation, including c-jun and the adenoviral oncoprotein E1A. The protein encoded by this gene associates with p300/CBP. It has in vitro and in vivo binding activity with CBP and p300, and competes with E1A for binding sites in p300/CBP. It has histone acetyl transferase activity with core histones and nucleosome core particles, indicating that this protein plays a direct role in transcriptional regulation. [provided by RefSeq]. 基因符号 - PCAF
- KAT2B
- CAF
- GCN5
- GCN5L
- P
- P/CAF
纯化方法 Affinity Purfied UniProt编号 - Q92831
UniProt汇总 FUNCTION: Functions as a histone acetyltransferase (HAT) to promote transcriptional activation. Has significant histone acetyltransferase activity with core histones (H3 and H4), and also with nucleosome core particles. Inhibits cell-cycle progression and counteracts the mitogenic activity of the adenoviral oncoprotein E1A. In case of HIV-1 infection, it is recruited by the viral protein Tat. Regulates Tat's transactivating activity and may help inducing chromatin remodeling of proviral genes.
CATALYTIC ACTIVITY: Acetyl-CoA + histone = CoA + acetylhistone.
SUBUNIT STRUCTURE: Interacts with SIRT1 By similarity. Interacts with EP300 and CREBBP. Interacts with NCOA1 and NCOA3. Component of a large chromatin remodeling complex, at least composed of MYSM1, KAT2B/PCAF, RBM10 and KIF11/TRIP5. Binds to HTLV-1 Tax. Interacts with and acetylates HIV-1 Tat. Interacts with KLF1; the interaction does not acetylate KLF1 and there is no enhancement of its transactivational activity. Interacts with NFE4.
SUBCELLULAR LOCATION: Nucleus By similarity UniProtKB Q92830.
TISSUE SPECIFICITY: Ubiquitously expressed but most abundant in heart and skeletal muscle.
DOMAIN: The bromodomain mediates binding to HIV-1 Tat.
SEQUENCE SIMILARITIES: Belongs to the GCN5 family.
Contains 1 bromo domain.
Contains 1 N-acetyltransferase domain.产品使用声明 质量保证 Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 0.5 µg/ml of this antibody detected P/CAF on 10 µg of A431 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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