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merck millipore,默克密理博,06-1084,Anti-IL-4R (CD124) Antibody, cytoplasmic
产品名称：Anti-IL-4R (CD124) Antibody, cytoplasmic
产品型号：06-1084
This Anti-IL-4R (CD124) Antibody, cytoplasmic is validated for use in ELISA, IC, IH(P), FC for the detection of IL-4R (CD124).
merck millipore,默克密理博,06-1084,Anti-IL-4R (CD124) Antibody, cytoplasmic
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Chp, H FC, ELISA, ICC Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 06-1084 描述 Anti-IL-4R (CD124) Antibody, cytoplasmic Alternate Names - interleukin-4 receptor alpha chain
- CD124 antigen
- interleukin 4 receptor
- interleukin 4 receptor alpha chain
背景信息 Interleukin 4 receptor (IL-4R) is a type I cytokine receptor that can bind interleukin 4 and interleukin 13 to regulate IgE antibody production in B cells. In T cells, IL-4R can also bind interleukin 4 to promote differentiation of Th2 cells. Soluble IL-4R can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. 产品信息 格式 Affinity Purified 控制 - Human tonsil tissue, human B and T lymphocytes
演示 Purified rabbit polyclonal in PBS with 0.09% sodium azide. 应用 应用 This Anti-IL-4R (CD124) Antibody, cytoplasmic is validated for use in ELISA, IC, IH(P), FC for the detection of IL-4R (CD124). 主要应用 - Flow Cytometry
- ELISA
- Immunocytochemistry
应用说明 Flow Cytometry Analysis: 5 µg from a previous lot detected IL-4R in human PBMCs.
Immunocytochemsitry: A previous lot of this antibody positively stained human B and T lymphocytes.
ELISA: A previous lot of this antibody was shown to work in ELISA by an independent laboratory.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human IL-4R at and around the cytoplasmic domain. 表位 Cytoplasmic Domain 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes the cyoplasmic domain of IL-4R. 品种反应性 ChimpanzeeHuman Species Reactivity Note Proven to react with human. Predicted to react with Chimpanzee based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_000409
Entrez基因汇总 This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by an alternate splice variant or by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Two transcript variants encoding different isoforms, a membrane-bound and a soluble form, have been found for this gene. [provided by RefSeq] 基因符号 - IL4RA
- IL4R
- CD124
- IL-4R-alpha
纯化方法 Affinity Purfied UniProt编号 - P24394
UniProt汇总 FUNCTION: Receptor for both interleukin 4 and interleukin 13. Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammmation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.
Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
SUBUNIT STRUCTURE: The functional IL4 receptor is formed by initial binding of IL4 to IL4R. Subsequent recruitment to the complex of the common gamma chain, in immune cells, creates a type I receptor and, in non-immune cells, of IL13RA1 forms a type II receptor. IL4R can also interact with the IL13/IL13RA1 complex to form a similar type II receptor. Interacts with PIK3C3 By similarity. Interacts with the SH2-containing phosphatases, PTPN6/SHIP1, PTPN11/SHIP2 and INPP5D/SHIP By similarity. Interacts with JAK1 through a Box 1-containing region. In Th1 cells, binds the N-terminal 50 amino acids of SOCS5, which inhibits JAK1 interaction, STAT6 activation and Th2 cell differentiation. SOCS5 interaction is independent of tyrosine phosphorylation By similarity.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein.
Isoform 2: Secreted.
TISSUE SPECIFICTY: Both isoform 1 and isoform 2 are highly expressed in activated T-cells.
DOMAIN: The extracellular domain represents the IL4 binding protein (IL4BP).
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.
Contains 1 copy of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-based inhibitor motif (ITIM). This motif is involved in modulation of cellular responses. The phosphorylated ITIM motif can bind the SH2 domain of several SH2-containing phosphatases.
PTM: On IL4 binding, phosphorylated on C-terminal tyrosine residues. Phosphorylation on any one of tyrosine residues, Tyr-575, Tyr-603 or Tyr-631, is required for STAT6-induced gene induction.
The soluble form (sIL4R/IL4BP) can also be produced by proteolytic cleavage at the cell surface (shedding) by a metalloproteinase.
POLYMORPHISM: Allelic variants in IL4RA are associated with a susceptibility to atopy, an immunological condition that can lead to clinical symptoms such as allergic rhinitis, sinusitis, asthma and eczema.
SEQUENCE SIMILARITIES: Belongs to the type I cytokine receptor family. Type 4 subfamily.
Contains 1 fibronectin type-III domain.产品使用声明 质量保证 Evaluated by immunohistochemistry in human tonsil tissue.
Immunohistochemistry (paraffin) Analysis: A 1:100 dilution of this antibody positively stained restricted lymphocytes in the corona and mantle cell regions of the tonsil follicles.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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