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merck millipore,默克密理博,05-824,Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal
产品名称：Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal
产品型号：05-824
Detect Estrogen Receptor β also known as Nuclear receptor subfamily 3 group A member 2 with Anti-Estrogen Receptor β Antibody, clone 68-4 (Rabbit Monoclonal Antibody), that has been demonstrated to wo More>>
merck millipore,默克密理博,05-824,Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal
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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB Rb Purified Monoclonal Antibody 描述 产品目录编号 05-824 品牌系列 Upstate
商名 - Upstate
描述 Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal 概述 Produced in collaboration with Epitomics 背景信息 Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. 产品信息 格式 Purified 控制 - MCF7 cell extracts
演示 Protein A Purified immunoglobulin in 0.1M Tris-Glycine, 0.15M NaCl and 0.05% sodium azide, pH 7.4. 应用 应用 Detect Estrogen Receptor β also known as Nuclear receptor subfamily 3 group A member 2 with Anti-Estrogen Receptor β Antibody, clone 68-4 (Rabbit Monoclonal Antibody), that has been demonstrated to work in IP & WB. 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated, synthetic peptide corresponding to amino acids 63-82 of rat and mouse estrogen receptor β and amino acids 55-74 of human estrogen receptor β 克隆 68-4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Estrogen Receptor β, long form, does not detect estrogen receptor alpha. 同种型 IgG 品种反应性 Human Species Reactivity Note Predicted to cross-react with rat and mouse based on sequence homology 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001437
Entrez基因汇总 This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. 基因符号 - ER-beta
- ESR2
- ESR-BETA
- ESTRB
- ESRB
- ER-BETA
- NR3A2
- Erb
纯化方法 Protein A purfied UniProt编号 - Q92731
UniProt汇总 FUNCTION: SwissProt: Q92731 # Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner. Isoform beta-cx lacks ligand binding ability and has no or only very low ere binding activity resulting in the loss of ligand-dependent transactivation ability. DNA- binding by ESR1 and ESR2 is rapidly lost at 37 degrees Celsius in the absence of ligand while in the presence of 17 beta-estradiol and 4-hydroxy-tamoxifen loss in DNA-binding at elevated temperature is more gradual.
SIZE: 530 amino acids; 59216 Da
SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with PELP1 and UBE1C. Isoform beta-2/cx preferentially forms a hetereodimer with ESR1 rather than ESR2 and inhibits DNA-binding by ESR1. Interacts with AKAP13.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Isoform beta-1 is expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. Isoform beta-2 is expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. Isoform beta-3 is found in testis. Isoform beta-4 is expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. Isoform beta-5 is expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain.DOMAIN:SwissProt: Q92731 Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily. & Contains 1 nuclear receptor DNA-binding domain.产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA lysates from MCF7 cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µL
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