merck millipore,默克密理博,05-768R,Anti-dimethyl-Histone H3 (Lys9) Antibody, rabbit monoclonal

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merck millipore,默克密理博,05-768R,Anti-dimethyl-Histone H3 (Lys9) Antibody, rabbit monoclonal
产品名称：Anti-dimethyl-Histone H3 (Lys9) Antibody, rabbit monoclonal
产品型号：05-768R
Use Anti-dimethyl-Histone H3 (Lys9) Antibody (Rabbit Monoclonal Antibody) validated in WB, PIA to detect dimethyl-Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di methyl K9).

merck millipore,默克密理博,05-768R,Anti-dimethyl-Histone H3 (Lys9) Antibody, rabbit monoclonal

产品介绍

merck millipore,默克密理博,05-768R,Anti-dimethyl-Histone H3 (Lys9) Antibody, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	WB	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	05-768R
描述	Anti-dimethyl-Histone H3 (Lys9) Antibody, rabbit monoclonal
Alternate Names	histone cluster 3, H3 H3K9me2 Histone H3 (di methyl K9) H3 histone family, member T histone 3, H3
背景信息	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.

产品信息
格式	Purified
控制	HeLa Acid Extract
演示	Purified Rabbit Monoclonal IgG Supernatant in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) containing 40% glycerol with 0.05% sodium azide.

应用
应用	Use Anti-dimethyl-Histone H3 (Lys9) Antibody (Rabbit Monoclonal Antibody) validated in WB, PIA to detect dimethyl-Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di methyl K9).
主要应用	Western Blotting

生物信息
免疫原品种	KLH- Conjugated - Linear Peptide corresponding to the N-terminus of Dimethyl-Histone H3 (Lys9). Methylated at and around Lysine 9
表位	Histone H3ethylated at Lysine 9
克隆	55-10C-1
宿主	Rabbit
特异性	Antibody recognizes Dimethyl-Histone H3 methylated at Lysine 9. Slight cross reactivity to mono and dimethyl histone H3 (Lys9).
同种型	IgG
品种反应性	Human
Species Reactivity Note	Demonstrated to react with human on HeLa acid extract. Broad specificity expected based on 100% sequence homology to multiple species.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_003493.2
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
基因符号	MGC126888 H3T H3/g H3.4 H3/t HIST3H3 H3FT MGC126886 H3t
修改	Methylation
纯化方法	Protein A purfied
UniProt编号	Q16695
UniProt汇总	Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Subunit structure: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Subcellular location: Nucleus. Tissue specificity: Expressed in testicular cells. Developmental stage: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. Post-translational modification: Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8sme2). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me) By similarity. Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription By similarity. Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8sme2) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters By similarity. Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin By similarity. Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation By similarity. Phosphorylation at Ser-11 (H3S10ph) is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at Ser-11 (H3S10ph) by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Ubiquitinated: By similarity. Sequence similarities: Belongs to the histone H3 family.

产品使用声明
质量保证	Evaluated by Western Blot in HeLa acid extract. Western Blot Analysis: 0.1 µg/ml of this antibody detected dimethyl Histone H3 (Lys9) in 10 µg of HeLa acid extract lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.