merck millipore,默克密理博,05-659,Anti-Kaiso Antibody, clone 6F

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merck millipore,默克密理博,05-659,Anti-Kaiso Antibody, clone 6F
产品名称：Anti-Kaiso Antibody, clone 6F
产品型号：05-659
Detect Kaiso with Anti-Kaiso Antibody, clone 6F (Mouse Monoclonal Antibody), that has been shown to work in EMSA, IP, WB & IHC.

merck millipore,默克密理博,05-659,Anti-Kaiso Antibody, clone 6F

产品介绍

merck millipore,默克密理博,05-659,Anti-Kaiso Antibody, clone 6F

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, Ca, Ch, H, Ht, M, Mk	IHC, EMSA, WB	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-659
品牌系列	Upstate
商名	Upstate
描述	Anti-Kaiso Antibody, clone 6F

产品信息
格式	Purified
演示	0.07M Tris-glycine, pH 7.4, 0.105M NaCl, 0.035% sodium azide and 30% glycerol.

应用
应用	Detect Kaiso with Anti-Kaiso Antibody, clone 6F (Mouse Monoclonal Antibody), that has been shown to work in EMSA, IP, WB & IHC.
主要应用	Immunohistochemistry Electrophoretic Mobility Shift Assay Western Blotting

生物信息
免疫原品种	Recombinant N-terminal GST and His-tagged fusion protein corresponding to residues 103-584 of murine Kaiso
克隆	clone 6F
宿主	Mouse
特异性	Kaiso
同种型	IgG1
品种反应性	RatCanineChickenHumanHamsterMouseMonkey
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_006777
基因符号	KAISO ZBTB33 ZNF348 kaiso ZNF-kaiso WUGSC:H_DJ525N14.1
纯化方法	Protein G chromatography
UniProt编号	Q86T24
UniProt汇总	FUNCTION: SwissProt: Q86T24 # Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3'. Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. SIZE: 672 amino acids; 74484 Da SUBUNIT: Self-associates. Interacts with CTNND2 (By similarity). Interacts with CTNND1, and this interaction inhibits binding to both methylated and non-methylated DNA. Interacts with NCOR1. Interacts with KPNA2/RCH1, which may mediate nuclear import of this protein. SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Also cytoplasmic in cells grown at high densities. TISSUE SPECIFICITY: Expressed in vascular endothelium. SIMILARITY: SwissProt: Q86T24 ## Contains 1 BTB (POZ) domain. & Contains 3 C2H2-type zinc fingers.

产品使用声明
质量保证	Immunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells. Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999). Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001). Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

产品使用声明

质量保证

Immunoblot Analysis: 0.5-2 μg/ml of this antibody detected Kaiso in RIPA lysates from 3T3/A31cells.
Immunohistochemistry: As shown by an independent laboratory, 1μg/ml of this antibody gave positive nuclear immunostaining for Kaiso in NIH 3T3 cells fixed with 100% methanol (Daniel, 2001; Daniel, 1999).
Immunoprecipitation: As shown by an independent laboratory, 2-5μg of this antibody immunoprecipitated Kaiso from RIPA lysates from NIH 3T3 cells (Daniel, 1999; Prokhortchouk, 2001).
Supershift: As shown by an independent laboratory, this antibody will supershift a specific methyl-CpG complex (Prokhortchouk, 2001).

使用声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.