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品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-572 Replaces 04-443 品牌系列 Upstate
商名 - Upstate
描述 Anti-Caspase 9 Antibody, clone 96-2-22 产品信息 格式 Purified 控制 - Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
演示 Protein A Purified immunoglobulin in Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative. 应用 应用 This Anti-Caspase 9 Antibody, clone 96-2-22 is validated for use in WB for the detection of Caspase 9. 主要应用 - Western Blotting
生物信息 免疫原品种 The N-terminal fragment of human Caspase 9 (amino acid residues 1-134) 克隆 96-2-22 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes the proform and the active cleaved form of Caspase 9. 同种型 IgG 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_032996
Entrez基因汇总 This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein is processed by caspase APAF1; this step is thought to be one of the earliest in the caspase activation cascade. Alternative splicing results in two transcript variants which encode different isoforms. 基因符号 - APAF-3
- CASP9
- CASP-9
- ICE-LAP6
- MCH6
- APAF3
- CASPASE-9c
- OTTHUMP00000044594
纯化方法 Protein G purfied UniProt编号 - P55211
UniProt汇总 FUNCTION: SwissProt: P55211 # Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9.
SIZE: 416 amino acids; 46281 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 35 kDa (p35) and a 10 kDa (p10) subunit. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome C and ATP. Interacts with the inhibitors BIRC2, BIRC4, BIRC5 and BIRC7.
TISSUE SPECIFICITY: Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues.
PTM: Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase- 3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.
SIMILARITY: SwissProt: P55211 ## Belongs to the peptidase C14 family. & Contains 1 CARD domain.产品使用声明 质量保证 routinely evaluated by immunoblot on RIPA lysates from A431, Raji, or HFF cells 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
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