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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 ABD78 描述 Anti-Dnmt3l Antibody Alternate Names - DNA (cytosine-5)-methyltransferase 3-like
背景信息 Methylation of DNA plays an important role in the regulation of gene expression in eukaryotic cells. This process relies on the activity of DNA methyltransferases, including DNMT3A and DNMT3B. The DNMT3L (DNA (cytosine-5)-methyltransferase 3-like) protein shares sequence homology with the DNMT3 family, but lacks a catalytic domain. DNMT3L regulates DNA methylation by binding to the catalytic domains of DNMT3A and DNMT3B thus enabling more rapid binding of these proteins to DNA and cofactors such as AdoMet. Previous studies have demonstrated that DNMT3L stimulates de novo methylation of DNA by activating murine Dnmt3L; and under those conditions, methylation was observed at specific maternal imprinting centers. 产品信息 格式 Affinity Purified 控制 - Mouse testis tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-Dnmt3l Antibody is validated for use in Western Blotting for the detection of Dnmt3l. 主要应用 - Western Blotting
应用说明 Western Blot Analysis: A representative lot of this antibody detected Dnmt3l in human testis tissue lysate, and in H9 and mESC cell lysates. 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to the domain of ADD of human Dnmt3l. 表位 Domain of ADD. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes domain of ADD of Dnmt3l. 品种反应性 MouseHuman Species Reactivity Note Demonstrated to react with Human and Mouse. Other homologies: Rat (83% sequence homology). 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_037501
Entrez基因汇总 CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1. Alternative splicing results in two transcript variants. An additional splice variant has been described but its biological validity has not been determined. 基因符号 - DNMT3L
纯化方法 Affinity purified UniProt编号 - Q9UJW3
UniProt汇总 FUNCTION: Catalytically inactive regulatory factor of DNA methyltransferases. It is essential for the function of DNMT3A and DNMT3B. Activates DNMT3A and DNMT3B by binding to their catalytic domain. Accelerates the binding of DNA and AdoMet to the methyltransferases and dissociates from the complex after DNA binding to the methyltransferases. Recognizes unmethylated histone H3 lysine 4 (H3K4) and induces de novo DNA methylation by recruitment or activation of DNMT3.
SUBUNIT STRUCTURE: Homodimer. Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with histone H3 (via N-terminus).
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Expressed at low levels in several tissues including testis, ovary, and thymus.
MISCELLANEOUS: Interaction with histone H3 is strongly inhibited by methylation at lysine 4 (H3K4me).
SEQUENCE SIMILARITIES: Belongs to the C5-methyltransferase family. Contains 1 ADD domain. Contains 1 GATA-type zinc finger. Contains 1 PHD-type zinc finger.产品使用声明 质量保证 Evaluated by Western Blot in mouse testis tissue lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected Dnmt3l in 10 µg of mouse testis tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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