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merck millipore,默克密理博,05-525,Anti-Rad50 Antibody, clone 13B3/2C6
产品名称：Anti-Rad50 Antibody, clone 13B3/2C6
产品型号：05-525
Anti-Rad50 Antibody, clone 13B3/2C6 is a high quality Mouse Monoclonal Antibody for the detection of Rad50 & has been validated in IP & WB.
merck millipore,默克密理博,05-525,Anti-Rad50 Antibody, clone 13B3/2C6
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-525 品牌系列 Upstate
商名 - Upstate
描述 Anti-Rad50 Antibody, clone 13B3/2C6 产品信息 格式 Purified 演示 10mM PBS, pH 7.4 应用 应用 Anti-Rad50 Antibody, clone 13B3/2C6 is a high quality Mouse Monoclonal Antibody for the detection of Rad50 & has been validated in IP & WB. 主要应用 - Western Blotting
生物信息 免疫原品种 GST fusion protein corresponding to residues 1-425 of human Rad50 克隆 13B3/2C6 宿主 Mouse 特异性 Rad50 同种型 IgG1κ 品种反应性 MouseHuman 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005732
Entrez基因汇总 The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. 基因符号 - RAD50-PEN
- RAD50
- RAD50-2
- hRad50
- hRAD50
纯化方法 Protein G chromatography UniProt编号 - Q92878
UniProt汇总 FUNCTION: SwissProt: Q92878 # Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand- specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point. The complex may also be required for DNA damage signaling via activation of the ATM kinase. In telomeres the MRN complex may modulate t-loop formation.
COFACTOR: Binds 1 zinc ion per homodimer (By similarity).
SIZE: 1312 amino acids; 153892 Da
SUBUNIT: Component of the MRN complex composed of two heterodimers RAD50/MRE11A associated with a single NBN. Component of the BASC complex, at least composed of BRCA1, MSH2, MSH6, MLH1, ATM, BLM, RAD50, MRE11A and NBN. Found in a complex with TERF2. Interacts with RINT1. Interacts with BRCA1 via its N-terminal domain. Interacts with DCLRE1C/Artemis.
SUBCELLULAR LOCATION: Nucleus. Note=Localizes to discrete nuclear foci after treatment with genotoxic agents.TISSUE SPECIFICITY: Expressed at very low level in most tissues, except in testis where it is expressed at higher level. Expressed in fibroblasts.
DOMAIN: SwissProt: Q92878 The zinc-hook, which separates the large intramolecular coiled coil regions, contains 2 Cys residues that coordinate one molecule of zinc with the help of the 2 Cys residues of the zinc- hook of another RAD50 molecule, thereby forming a V-shaped homodimer. The two heads of the homodimer, which constitute the ATP-binding domain, interact with the MRE11A homodimer (By similarity).
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).
SIMILARITY: Belongs to the SMC family. RAD50 subfamily. & Contains 1 zinc-hook domain.
MISCELLANEOUS: In case of infection by adenovirus E4, the MRN complex is inactivated and degraded by viral oncoproteins, thereby preventing concatenation of viral genomes in infected cells.产品使用声明 质量保证 routinely evaluated by immunoblot on HeLa nuclear lysate 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 100 µg
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