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品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1500 描述 Anti-Mad1 Antibody, clone 9B10 Alternate Names - mitotic-arrest deficient 1, yeast, homolog-like 1
- tumor protein p53 inducible protein 9
- MAD1 (mitotic arrest deficient, yeast, homolog)-like 1
- MAD1 mitotic arrest deficient-like 1 (yeast)
- MAD1-like 1
- MAD1-like 1 protein
- Mitotic arrest deficient-like protein 1
- Mitotic checkpoint MAD1 protein-homolog
- Tax-binding protein 181
- Tax-binding protein 181
- mitotic-arrest deficient 1
- homolog-like 1
背景信息 Mitotic arrest deficient-like 1 (yeast) is also known as MAD1 and MAD1L1. It is part of the MAD1 family which is crucial to development. MAD1 acts as a checkpoint gene during mitotic spindle assembly. During anaphase and telophase MAD1 is located at the spindle mid-zone of the cell, where it prevents anaphase until the chromosome is aligned at the metaphase plate. During metaphase MAD1 is located at the centrosome. MAD1 participates in cell cycle control and tumor suppression. MAD1 functions as a homodimer and interacts with MAD2L1. It is thought that MAD1 recruits to MAD2, which then promotes binding of MAD2 to CDC20. 产品信息 格式 Purified 控制 - HeLa nuclear extract
演示 Purified mouse monoclonal IgG2b in buffer containing 0.1 M Tris-Glycine, (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Mad1 Antibody, clone 9B10 is an antibody against Mad1 for use in WB, IC & IP. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunoprecipitation
Immunocytochemistry生物信息 免疫原品种 Full length recombinant Mad1. 克隆 9B10 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Mad1. 同种型 IgG2bκ 品种反应性 Human Species Reactivity Note Tested on Human cells. 抗体类型 Monoclonal Antibody 基因符号 - hMAD1
- HsMAD1
- MAD1
- PIG9
- TP53I9
- TXBP181
纯化方法 Protein G purfied UniProt编号 - Q9Y6D9
UniProt汇总 FUNCTION: Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Ref.2
STRUCTURE:Homodimer. Heterodimerizes with MAD2L1 in order to form a tetrameric MAD1L1-MAD2L1 core complex. Perturbation of the original MAD1L1-MAD2L1 structure by the spindle checkpoint may decrease MAD2L1 affinity for MAD1L1. CDC20 can compete with MAD1L1 for MAD2L1 binding, until the attachment and/or tension dampen the checkpoint signal, preventing further release of MAD2L1 on to CDC20. Also able to interact with the BUB1/BUB3 complex and the viral Tax protein. Ref.1
SUBCELLULAR LOCATION: Nucleus. Note= From the beginning to the end of mitosis, it is seen to move from a diffusely nuclear distribution to the centrosome, to the spindle midzone and finally to the midbody. Ref.1
TISSUE SPECIFICITY: Expressed weakly at G0/G1 and highly at late S and G2/M phase.
Induction Increased by TP53. Ref.2
PTM: Phosphorylated; by BUB1. Become hyperphosphorylated in late S through M phases or after mitotic spindle damage. Phosphorylated upon DNA damage, probably by ATM or ATR. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13
DISEASE: Defects in MAD1L1 are involved in the development and/or progression of various types of cancer.
SIMILARITY: Belongs to the MAD1 family.
SEQUENCE CAUTION: The sequence AAC52059.1 differs from that shown. Reason: Frameshift at position 663.
产品使用声明 质量保证 Western Blot Analysis:
1-5 µg/mL of this anti-Mad1, clone 9B10 detected Mad1 in HeLa nuclear extract.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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