merck millipore,默克密理愽
  • merck millipore,默克密理博,05-345,Anti-p21/WAF1/Cip1 Antibody

  • merck millipore,默克密理博,05-345,Anti-p21/WAF1/Cip1 Antibody

    产品名称：Anti-p21/WAF1/Cip1 Antibody
    产品型号：05-345
    Use Anti-p21/WAF1/Cip1 Antibody (Mouse Monoclonal Antibody) validated in WB, IP to detect p21/WAF1/Cip1 also known as CDK-interacting protein or cyclin-dependent kinase inhibitor 1A.

    merck millipore,默克密理博,05-345,Anti-p21/WAF1/Cip1 Antibody

  • 产品介绍
  • merck millipore,默克密理博,05-345,Anti-p21/WAF1/Cip1 Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    M, HWBMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-345
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-p21/WAF1/Cip1 Antibody
    Alternate Names
    • wild-type p53-activated fragment 1
    • CDK-interacting protein
    • CDK-interaction protein
    • DNA synthesis inhibitor
    • Melanoma differentiation-associated protein 6
    • cyclin-dependent kinase inhibitor 1A
    • melanoma differentiation associated protein 6
    背景信息 Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically. The biochemical activities and patterns of expression of CKIs during development implicate these proteins as the primary effectors of signaling pathways that control cell cycle exit, an event that is critical for differentiation. Studies have shown that p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is a relatively poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb, which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.
    产品信息
    格式 Purified
    控制
    • Included Positive Antigen: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels.
    演示 Purified, pooled monoclonal mouse IgGs in buffer containing 0.1 M Trisglycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.
    应用
    应用 Use Anti-p21/WAF1/Cip1 Antibody (Mouse Monoclonal Antibody) validated in WB, IP to detect p21/WAF1/Cip1 also known as CDK-interacting protein or cyclin-dependent kinase inhibitor 1A.
    主要应用
    • Western Blotting
    应用说明 Immunoprecipitation: 4 μg of a previous lot immunoprecipitated Cip1 from 500 μg of HeLa nuclear extract.
    生物信息
    免疫原品种 Recombinant full-length human Cip1 with a histidine tag.
    表位 amino acids 1-80
    克隆 CP36, CP74
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Recognizes Cip1, MW 21 kDa
    同种型 IgG
    品种反应性 MouseHuman
    Species Reactivity Note Human and mouse; other species cross-reactivity is unknown.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_000389
    Entrez基因汇总 This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.
    基因符号
    • CAP20
    • CDKN1A
    • MDA-6
    • SDI1
    • MDA6
    • p21
    • p21CIP1
    • P21
    • CIP1
    • PIC1
    • CDKN1
    • WAF1
    纯化方法 Protein G purfied
    UniProt编号
    • P38936
    UniProt汇总 FUNCTION: SwissProt: P38936 # May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.
    SIZE: 164 amino acids; 18119 Da
    SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
    TISSUE SPECIFICITY: Expressed in all adult human tissues, with 5- fold lower levels observed in the brain.
    PTM: Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA.
    SIMILARITY: SwissProt: P38936 ## Belongs to the CDI family.
    产品使用声明
    质量保证 Evaluated by western blotting on HeLa nuclear extract.

    Western Blot Analysis: 0.5-2 μg/mL of this antibody detected Cip1 in 20 μg of HeLa nuclear extract
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at from date of receipt.
    包装信息
    数量 200 µg

    merck millipore,默克密理博,05-345,Anti-p21/WAF1/Cip1 Antibody

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