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品种反应性 主要应用 宿主 格式 抗体类型 R, H WB, ELISA, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-166 品牌系列 Upstate
商名 - Upstate
描述 Anti-IGF-II Antibody, clone S1F2 背景信息 Human Insulin-like Growth Factor II (IGFII) contains 67 amino acids and shares similar structural features with IGFI, including a 62% sequence homology. In human plasma, IGFI and IGFII are associated with IGF-binding proteins that transport the polypeptides and partially regulate their actions in vivo. In addition to the insulin receptor, IGFII binds to two forms of IGF receptors, both of which are widely distributed in different tissues and cultured cells. IGFII is mitogenic for a variety of cultured cells, including mouse 3T3 cells,12 normal rat kidney cells, human or chicken fibroblasts and MCF-7 human breast carcinoma cells. 产品信息 格式 Purified 控制 - Human head and neck tumours, angioma tissues
演示 Purified mouse monoclonal IgG1 in buffer containing PBS, pH 7.4., purified by ammonium sulfate precipitation and DEAE-cellulose chromatography. Frozen solution. 应用 应用 Detect IGF-II using this Anti-IGF-II Antibody, clone S1F2 validated for use in ELISA, IP, IC, NEUT & WB. 主要应用 - Western Blotting
- ELISA
- Immunocytochemistry
应用说明 Neutralization: 10 μg/mL of a previous lot inhibited the stimulatory effects of IGF-II (10 ng/mL) on DNA synthesis in chicken embryo fibroblasts. Cell viability was assessed using an ATP endpoint assay (ATPLiteÔ-M, Packard Instruments). Previous lots of antibody were tested to inhibit IGF-II-dependent incorporation of [3H]-thymidine 生物信息 免疫原品种 Partially purified rat IGF-II. Clone S1F2 克隆 S1F2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody detects IGF-II. 同种型 IgG1 品种反应性 RatHuman Species Reactivity Note Recognizes rat and human IGF-II. Less than 10% cross-reactivity to human IGF-I (Tanaka, H, 1989; Suzuki, T, 1989). 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_000612
Entrez基因汇总 This gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is an imprinted gene and is expressed only from the paternally inherited allele. It is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. 基因符号 - C11orf43
- IGF2
- FLJ22066
- FLJ44734
- pp9974
- IGF-II
- INSIGF
纯化方法 Ammonium sulfate precipitation and DEAE-cellulose chromatography UniProt编号 - P01344
UniProt汇总 FUNCTION: SwissProt: P01344 # The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
SIZE: 180 amino acids; 20140 Da
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: SwissProt: P01344 ## Belongs to the insulin family.产品使用声明 质量保证 Evaluated by Western Blotting on HeLa acid extracted nuclear preps (positive) and recombinant Histone H3 (negative).
Western Blotting Analysis:
1:5,000 – 1:10,000 dilution of this antibody was used to detect trimethylated-Histone H3 (Lys4) in HeLa nuclear acid extracts.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 500 µg
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