merck millipore,默克密理愽
  • merck millipore,默克密理博,AB5382,Anti-Nitric Oxide Synthase II Antibody

  • merck millipore,默克密理博,AB5382,Anti-Nitric Oxide Synthase II Antibody

    产品名称：Anti-Nitric Oxide Synthase II Antibody
    产品型号：AB5382
    Anti-Nitric Oxide Synthase II Antibody is an antibody against Nitric Oxide Synthase II for use in IC, IH & WB.

    merck millipore,默克密理博,AB5382,Anti-Nitric Oxide Synthase II Antibody

  • 产品介绍
  • merck millipore,默克密理博,AB5382,Anti-Nitric Oxide Synthase II Antibody

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    R, MWB, ICC, IHCRbSerumPolyclonal Antibody
    描述
    产品目录编号 AB5382
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Nitric Oxide Synthase II Antibody
    Alternate Names
    • iNOS
    • NOS II
    背景信息 Nitric Oxide Synthases are the only enzymes know to simultaneously require five bound cofactors, FAD, FMN, haem, tetrahydrobiopterin (BH4) and Ca++-Calmodulin (CAM). In mammals, there are three distinct genes for NOS, NOS I is neuronal, NOS II is cytokine inducible, and NOS III is endothelial. The enzymes exist as homodimers, each monomer consisting of two major domains: N­terminal oxygenase domain, which belongs to the class of haem-thiolate proteins, and C­terminal reductase domain, which is homologous to NADPH:P450 reductase. The interdomain linker between the oxygenase and reductase domains contains a CaM­binding sequence. Functionally NOS is an enzyme that catalyzes the stepwise conversion of the amino acid L-arginine to nitric oxide and L-citrulline. NOS activity stimulates cyclic GMP, inducing smooth muscle relaxation by increasing intracellular cGMP, which in turn inhibits calcium entry into the cell, and decreases intracellular calcium concentrations. This also stimulates a cGMP-dependent protein kinase that activates myosin light chain phosphatase, the enzyme that dephosphorylates myosin light chains, which leads to smooth muscle relaxation.
    产品信息
    格式 Serum
    控制
    • Macrophages, liver lysates
    演示 Rabbit polyclonal serum in buffer containing liquid 0.05% sodium azide.
    应用
    应用 Anti-Nitric Oxide Synthase II Antibody is an antibody against Nitric Oxide Synthase II for use in IC, IH & WB.
    主要应用
    • Western Blotting
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunohistochemistry:
    1:5000 (ABC) dilution of a previous lot was used on tissue sections (rat liver).

    Immunocytochemistry:
    1:50,000 dilution of a previous lot was used on cultured cells.

    Western blot:
    1:5,000 (ECL). The antibody has been used successfully on rat liver. It is not recommended for use on rat brain extracts since unidentified bands may be present.

    Optimal working dilutions must be determined by end user.
    生物信息
    免疫原品种 C-terminal 14 amino acid peptide from mouse macrophage nitric oxide synthase.
    表位 C-terminus
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Rabbit
    特异性 Inducible nitric oxide synthase (iNOS, NOS-II). No cross-reaction with rat nNOS and eNOS by Western blot and immunohistochemistry.
    品种反应性 RatMouse
    Species Reactivity Note Mouse and rat. Reactivity to human iNOS is approximately 1%.
    抗体类型 Polyclonal Antibody
    Entrez基因编号
    • NM_153292.1
    Entrez基因汇总 Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
    基因符号
    • EC 1.14.13.39
    • NOS2A
    • iNOS
    • HEP-NOS
    • INOS
    • NOS
    • NOS2
    纯化方法 Unpurified
    UniProt编号
    • P35228
    UniProt汇总 FUNCTION: SwissProt: P35228 # Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions.
    COFACTOR: Heme group (By similarity). & Binds 1 FAD (By similarity). & Binds 1 FMN (By similarity). & Metrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity).
    SIZE: 1153 amino acids; 131117 Da
    SUBUNIT: Homodimer. Binds SLC9A3R1.
    TISSUE SPECIFICITY: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not expressed in the platelets.
    SIMILARITY: SwissProt: P35228 ## Belongs to the NOS family. & Contains 1 flavodoxin-like domain.
    产品使用声明
    质量保证 Evaluated by Western Blot on Mouse liver lysates.

    Western Blot Analysis:
    1:1000 dilution of this antibody detected INOS on 10 µg of Mouse liver lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 6 months at -20°C in undiluted aliquots from date of receipt.
    Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 50 µL

    merck millipore,默克密理博,AB5382,Anti-Nitric Oxide Synthase II Antibody

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