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Replacement Information Replacement Information 05-1427 is a recommended replacement for MAB3779 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ELISA, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1427 Replaces MAB3779 描述 Anti-ARF1 Antibody, clone 3F1 Alternate Names - ADP-ribosylation factor 1
背景信息 ARF1 is a member of the ARF family of small G proteins and is a component of Golgi-derived non-clathrin coated vesicles. This antibody is class 1 ARF specific. 产品信息 格式 Purified 控制 - HeLa cell lysate
演示 Purified mouse monoclonal IgG2a in 0.02 M phosphate buffer, 0.25 M NaCl, pH 7.6, with 0.1% sodium azide 应用 应用 Anti-ARF1 Antibody, clone 3F1 detects level of ARF1 & has been published & validated for use in WB, ELISA, IH(P). 主要应用 - ELISA
- Western Blotting
应用说明 ELISA: A previous lot of this antibody was used in ELISA.
Optimal working dilutions must be determined by end user.
Immunohistochemistry(paraffin): Representative testing from a previous lot.
Optimal Staining of ARF1 Monoclonal Antibody: Skin & Breast Cancer
生物信息 免疫原品种 Synthetic peptide corresponding to the C-terminal amino acid sequence of human ARF1. 表位 C-terminus 克隆 3F1 宿主 Mouse 特异性 Recognizes human ARF1 同种型 IgG2a 品种反应性 RatHumanMouse Species Reactivity Note Human, mouse and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001024226.1
Entrez基因汇总 ADP-ribosylation factor 1 (ARF1) is a member of the human ARF gene family. The family members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking as activators of phospholipase D. The gene products, including 6 ARF proteins and 11 ARF-like proteins, constitute a family of the RAS superfamily. The ARF proteins are categorized as class I (ARF1, ARF2 and ARF3), class II (ARF4 and ARF5) and class III (ARF6), and members of each class share a common gene organization. The ARF1 protein is localized to the Golgi apparatus and has a central role in intra-Golgi transport. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. 基因符号 - ARF1
纯化方法 Purified UniProt编号 - P84077
UniProt汇总 FUNCTION: SwissProt: P84077 # GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP- ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
SIZE: 181 amino acids; 20697 Da
SUBUNIT: Interacts with PRKCABP, GGA1, DDEF2 and PIP5K1A (By similarity). Interacts with ARFGAP1, which hydrolyzes GTP and thus, regulates its function. Binds DDEF2. Interacts with ARHGAP21.
SIMILARITY: SwissProt: P84077 ## Belongs to the small GTPase superfamily. Arf family产品使用声明 质量保证 Routinely evaluated by Western Blot on HeLa lysates.
Western Blot Analysis: 1:500 dilution of this lot detected ARF1 on 10 μg of HeLa lysates.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable at 2-8°C in undiluted aliquots for up to 1 year from date of receipt. 包装信息 数量 100 µg
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