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merck millipore,默克密理博,FCMAB124A4,Milli-Mark™ Anti-mOCT4 Antibody, clone 7F9.2, Alexa Fluor 488 Conjugate
产品名称：Milli-Mark™ Anti-mOCT4 Antibody, clone 7F9.2, Alexa Fluor 488 Conjugate
产品型号：FCMAB124A4
Detect mOCT4 using this Milli-Mark Anti-mOCT4 Antibody, clone 7F9.2, Alexa Fluor 488 Conjugate validated for use in FC.
merck millipore,默克密理博,FCMAB124A4,Milli-Mark™ Anti-mOCT4 Antibody, clone 7F9.2, Alexa Fluor 488 Conjugate
- 产品介绍
- merck millipore,默克密理博,FCMAB124A4,Milli-Mark™ Anti-mOCT4 Antibody, clone 7F9.2, Alexa Fluor 488 Conjugate
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H, M FC M AlexaFluor®488 Monoclonal Antibody 描述 产品目录编号 FCMAB124A4 商名 - Milli-Mark
描述 Milli-Mark™ Anti-mOCT4 Antibody, clone 7F9.2, Alexa Fluor® 488 Conjugate Alternate Names - Octamer-binding transcription factor 3 <br />POU class 5 homeobox 1 <br />POU domain class 5, transcription factor 1 <br />POU-type homeodomain-containing DNA-binding protein <br />octamer-binding transcription factor-3
背景信息 Octamer-4 (Oct-4), a member of the POU family of transcription factors, has been demonstrated to be vital for the formation of self-renewing pluripotent stem cells. During embryogenesis, expression of Oct-4 is limited to pluripotent cells of the inner cell mass (ICM) that contribute to the formation of all fetal cell types. This relationship between Oct-4 and pluripotency has seen this transcription factor emerge as a marker of pluripotent stem cells. Undifferentiated human and murine pluripotent Embryonic Stem (ES) and Embryonic Carcinoma (EC) cells express Oct-4. Additionally, murine Embryonic Germ (EG) cells are also known to express Oct-4. Following stem cell differentiation, the level of Oct-4 expression decreases. Oct-4 expression by human EG cells is not known. 产品信息 格式 AlexaFluor®488 控制 - Mouse embryonic stem cells.
演示 Purified mouse monoclonal IgG1κ conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA. 应用 应用 Detect mOCT4 using this Milli-Mark Anti-mOCT4 Antibody, clone 7F9.2, Alexa Fluor 488 Conjugate validated for use in FC. 主要应用 - Flow Cytometry
应用说明 Working Solution Cells / test Total Reaction Volume 5 μl 5 x 105 Cells / 95 μl PBS 100 μl 10 μl 1 x 106 Cells / 90 μl PBS 100 μl 生物信息 免疫原品种 Recombinant Protein corresponding to the N-terminus of human OCT-4 [POU5F1]. 表位 N-terminus 克隆 7F9.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Antibody recognizes OCT-4 [POU5F1]. N-terminus. 同种型 IgG1κ 品种反应性 HumanMouse Species Reactivity Note Proven to work on Mouse. Predicted to react on Human based on 100% sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_976034
基因符号 - Oct-4
- OCT3
- OCT4
- OTF3
- OTF4
- Oct-3
纯化方法 Protein G purfied UniProt编号 - P20263
UniProt汇总 FUNCTION: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.
SUBUNIT STRUCTURE:Interacts with UBE2I.
SUBCELLULAR LOCATION: Nucleus. Note= Expressed in a diffuse and slightly punctuate pattern.
TISSUE SPECIFICTY: Expressed the totipotent and pluripotent stem cells of the pregastrulation embryo. Also expressed in primordial germ cells and in the female germ line. Absent from adult tissues.
DEVELOPMENTAL STAGE: Down-regulated during differentiation to endoderm and mesoderm. Ref.1
INDUCTION: Repressed by retinoic acid (RA).
PTM: Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression.
SEQUENCE SIMILARITIES: Belongs to the POU transcription factor family. Class-5 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. ...产品使用声明 质量保证 Evaluated by flow cytometry using mouse embryonic stem cells. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt. 包装信息 数量 100 tests
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