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merck millipore,默克密理博,05-1327,Anti-PHD2 Antibody, clone 76a
产品名称：Anti-PHD2 Antibody, clone 76a
产品型号：05-1327
Use Anti-PHD2 Antibody, clone 76a (mouse monoclonal antibody) validated in WB to detect PHD2 also known as EGL nine (C.elegans) homolog 1, HIF prolyl hydroxylase 2, Hypoxia-inducible factor prolyl hyd More>>
merck millipore,默克密理博,05-1327,Anti-PHD2 Antibody, clone 76a
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1327 描述 Anti-PHD2 Antibody, clone 76a Alternate Names - egl nine-like protein 1
- egl nine homolog 1 (C. elegans)
- egl nine homolog 1
- Prolyl hydroxylase domain-containing protein 2
- Hypoxia-inducible factor prolyl hydroxylase 2
- HIF-prolyl hydroxylase 2
- HIF prolyl hydroxylase 2
- EGL nine (C.elegans) homolog 1
背景信息 PHD2 (Prolyl hydroxylase domain-containing protein 2), also known as Hypoxia-inducible factor prolyl hydroxylase 2 (HIF-PH2 or HPH-2), is one of 4 PHD (PHD1-4) proteins which function as prolyl hydroxylases to effect oxygen homeostasis via hypoxia-inducible factor (HIF). This transcriptional complex is composed of an alpha-beta heterodimer that mediates a broad range of cellular and systemic responses to hypoxia. The PHD proteins hydroxylate HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. The PHD2 isoform is the main down-regulator of HIFs for normal oxygen levels and mild hypoxia. When oxygen levels are normal, PHDs catalyze the hydroxylation of prolyl residues on HIF-1alpha. This post-translational modification targets this protein for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. 产品信息 格式 Purified 控制 - MCF-7 cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-PHD2 Antibody, clone 76a (mouse monoclonal antibody) validated in WB to detect PHD2 also known as EGL nine (C.elegans) homolog 1, HIF prolyl hydroxylase 2, Hypoxia-inducible factor prolyl hydroxylase 2. 主要应用 - Western Blotting
生物信息 免疫原品种 Recombinant protein corresponding to human PHD2. 表位 Unknown 克隆 76a 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes PHD2. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_071334.1
基因符号 - ZMYND6
- EGLN1
- C1orf12
- ECYT3
- HIF-PH2
- HIFPH2
- HPH-2
- HPH2
- PHD2
- SM-20
- SM20
纯化方法 Protein G purfied UniProt编号 - Q9GZT9
UniProt汇总 FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
CATALYTIC ACTIVITY: An HIF alpha chain L-proline + 2-oxoglutarate + O2 = An HIF alpha chain trans-4-hydroxy-L-proline + succinate + CO2.
COFACTOR: Binds 1 Fe2+ ion per subunit.
Ascorbate.
ENZYME REGULATION: Following exposure to hypoxia, activated in HeLa cells but not in cardiovascular cells. Seems to be inhibited by ING4.
SUBUNIT STRUCTURE: Monomer. Interacts with ING4.
TISSUE SPECIFICITY: According to Ref.1 widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to Ref.8 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle.
INVOLVEMENT IN DISEASE: Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.
SEQUENCE SIMILARITIES: Contains 1 MYND-type zinc finger.
Contains 1 PKHD (prolyl/lysyl hydroxylase) domain.
CAUTION: It was previously reported that this protein was the ortholog of rat SM-20. However, EGLN3 is now considered the true ortholog of rat SM-20 since it shows substantially greater similarity.
SEQUENCE CAUTION: The sequence AAK07536.1 differs from that shown. Reason: Frameshift at position 239.产品使用声明 质量保证 Evaluated by Western Blot in MCF-7 cell lysate.
Western Blot Analysis: 2 µg/ml of this antibody detected PHD2 on 10 µg of MCF-7 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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