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merck millipore,默克密理博,05-1321,Anti-BMI1 Antibody, clone AF27
产品名称：Anti-BMI1 Antibody, clone AF27
产品型号：05-1321
Anti-BMI1 Antibody, clone AF27 is a mouse monoclonal antibody for detection of BMI1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog & has been validate More>>
merck millipore,默克密理博,05-1321,Anti-BMI1 Antibody, clone AF27
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ChIP M Purified Monoclonal Antibody 描述 产品目录编号 05-1321 描述 Anti-BMI1 Antibody, clone AF27 Alternate Names - polycomb group ring finger 4
- B lymphoma Mo-MLV insertion region 1 homolog
- B lymphoma Mo-MLV insertion region 1 homolog (mouse)
- BMI1 polycomb ring finger oncogene
- Polycomb group RING finger protein 4
- RING finger protein 51
- murine leukemia viral (bmi-1) oncogene homolog
- oncogene BMI-1
- polycomb group protein Bmi1
背景信息 The BMI1 protein is a member of the polycomb group (PcG) proteins. PcG proteins form multiprotein complexes that function as transcriptional repressors. Several lines of evidence implicate BMI1 in tumorigenesis. BMI1 cooperates with c-Myc in the generation of lymphomas in double transgenic mice. BMI1 blocks senescence and immortalizes mouse embryo fibroblasts, although not human fibroblasts, and in combination with an activated H-ras gene, leads to neoplastic transformation. The BMI1 gene is amplified in certain mantle cell lymphomas and is overexpressed in a subset of non-small cell lung cancer, colorectal carcinomas, multiple myelomas and medulloblastomas, but not glioblastomas. The transformation of human hematopoietic progenitor cells by the oncogenic fusion protein E2a-Pbx1 requires BMI1. 产品信息 格式 Purified 控制 - MEF1 cell lysate
演示 Purified mouse monoclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-BMI1 Antibody, clone AF27 is a mouse monoclonal antibody for detection of BMI1 also known as BMI1 polycomb ring finger oncogene, murine leukemia viral (bmi-1) oncogene homolog & has been validated in IP, ChIP. 主要应用 - Chromatin Immunoprecipitation (ChIP)
应用说明 Chromatin Immunoprecipitation (ChIP):
A previous lot was used by an independent laboratory in ChIP. (Bracken, A.P., et al., 2007).生物信息 免疫原品种 MBP-tagged recombinant protein corres-ponding to full length human BMI1. 克隆 AF27 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes BMI1. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005171
基因符号 - flvi-2/bmi-1
- MGC12685
- PCGF4
- RNF51
纯化方法 Anti-BMI1, clone AF27 UniProt编号 - P35226
UniProt汇总 FUNCTION: Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
SUBUNIT STRUCTURE: Component of chromatin-associated class II polycomb repressive complex 1 (PRC1/hPRC-H) at least composed of PCGF2/RNF110, BMI1/PCGF4, CBX2/M33, CBX4/PC2, CBX8/PC3, PHC1, PHC2, PHC3, SCMH1, RING1 and RNF2/RING2. Also component of E3 ubiquitin ligase complex or human polycomb repressive complex 1-like at least composed of PHC2, BMI1, RING1 and RNF2. Interacts with SPOP. Part of a complex consisting of BMI1, CUL3 and SPOP. Interacts with E4F1.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm.
PTM: May be polyubiquitinated; which does not lead to proteasomal degradation.
INVOLVEMENT IN DISEASE: Cooperates with the MYC oncogene to produce B-lymphomas.
SEQUENCE SIMILARITIES: Contains 1 RING-type zinc finger.产品使用声明 质量保证 Evaluated by Immunoprecipitation in MEF1 cell lysate.
Immunoprecipitation Analysis: 10 μg/mL of this antibody immunoprecipitated BMI1 from 100 μg of MEF1 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable at 2-8°C for 1 year from date of receipt. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,AP501R,Goat Anti-Mouse IgM+IgG+IgA (H+L) Antibody, TRITC conjugate, Species Adsorbed
下一件merck millipore产品：merck millipore,默克密理博,70-620,NFκB p65 EZ-TFA Transcription Factor Assay
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