merck millipore,默克密理博,05-1249-S,Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307, Trial Size

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merck millipore,默克密理博,05-1249-S,Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307, Trial Size
产品名称：Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307, Trial Size
产品型号：05-1249-S
Use Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307 (mouse monoclonal antibody) validated in WB, ELISA, ICC, IP, Mplex to detect Dimethyl Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di More>>

merck millipore,默克密理博,05-1249-S,Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307, Trial Size

产品介绍

merck millipore,默克密理博,05-1249-S,Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307, Trial Size

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
Vrt, H	ELISA, WB, ChIP, ICC	M	Purified	Monoclonal Antibody

描述
产品目录编号	05-1249-S
描述	Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307, Trial Size
Alternate Names	histone cluster 3, H3 H3K4me2 Histone H3 (di methyl K4) H3 histone family, member T histone 3, H3
背景信息	Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. Histone proteins are highly post-translationally modified, and Histone H3 is the most extensively modified of the five histones. Histone H3 sequence variants and variable modification states are thought to play a role in the dynamic and long term regulation of genes. Di- and tri-methylation of histone H3 are gene silencing marks, while removal of these marks by the demethylase genes Jmjd1a and Jmjd2c leads to gene activation.

产品信息
格式	Purified
控制	HeLa acid extract
演示	Mouse monoclonal IgG1κ supernatant in PBS with 0.05% sodium azide.

应用
应用	Use Anti-Dimethyl Histone H3 (Lys9) Antibody, clone CMA307 (mouse monoclonal antibody) validated in WB, ELISA, ICC, IP, Mplex to detect Dimethyl Histone H3 (Lys9) also known as H3K9me2, Histone H3 (di methyl K9).
主要应用	ELISA Western Blotting Chromatin Immunoprecipitation (ChIP) Immunocytochemistry
应用说明	Chromatin Immunoprecipitation:: Sonicated chromatin prepared from HeLa cells (1 X 106 cell equivalents per IP) were subjected to chromatin immunoprecipitation using 2 µg of either a normal mouse IgG, or Anti-dimethyl Histone H3 (Lys9) antibody, clone CMA307 and the Magna ChIP G (Cat. # 17-611) Kit. Successful immunoprecipitation of dimethyl-histone H3 (Lys9) associated DNA fragments was verified by qPCR using ChIP Primers B-Globin. Please refer to the EZ-Magna G ChIP™ (Cat. # 17-409) or EZ-ChIP™ (Cat. # 17-371) protocol for experimental details. Dot Blot Analysis: Absurance Histone H3 Antibody Specificity Array (Cat. No. 16-667) and Absurance Histone H2A, H2B, H4 Antibody Specificity Array (Cat. No. 16-665), which contain histone peptides with various modifications were probed with Cat. No 05-1249-S, Anti-dimethyl H3 (Lys9), clone CMA307 at 2.0ug/mL (1:500) dilution. Proteins were visualized using a Donkey anti-mouse IgG conjugated to HRP and a chemiluminescence detection system. ELISA: This antibody has been shown by an outside laboratory to be suitable for ELISA. Kimura, H., et al. (2008). Cell Struct. Funct. 33: 61-73. Immunocytochemistry: This antibody has been shown by an outside laboratory to be suitable for immunocytochemistry ( Kimura, 2008). Immunoprecipitation: This antibody has been shown by an outside laboratory to be suitable for immunoprecipitation ( Kimura, 2008). Multiplexing: This antibody specifically recognizes histone H3 dimethylated on Lys9 by Luminex® assay.

生物信息
免疫原品种	Synthetic peptide corresponding to amino acids 1-19 of human Histone H3, dimethylated on Lys9, conjugated to KLH.
表位	dimethyl Lys9
克隆	CMA307
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Mouse
特异性	This antibody recognizes Histone H3 dimethylated at Lys9. Phosphorylation of Ser10 interferes with antibody binding to dimethyl Lys9.
同种型	IgG1κ
品种反应性	VertebratesHuman
Species Reactivity Note	Human. Broad species cross-reactivity is expected, based on sequence homology.
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_003493
Entrez基因汇总	Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq]
基因符号	OTTHUMP00000037945 H3.4 H3/g H3/t H3FT H3T H3t MGC126886 MGC126888
修改	Methylation
纯化方法	Protein G purfied
UniProt编号	Q16695
UniProt汇总	FUNCTION: SwissProt: Q16695 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. SIZE: 136 amino acids; 15508 Da SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. SUBCELLULAR LOCATION: Nucleus. TISSUE SPECIFICITY: Expressed in testicular cells. DEVELOPMENTAL STAGE: Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation. PTM: Acetylation is generally linked to gene activation. Acetylation on Lys-10 impairs methylation at Arg-9. Acetylation on Lys-19 and Lys-24 favors methylation at Arg-18 (By similarity). & Citrullination at Arg-9 and/or Arg-18 by PADI4 impairs methylation and represses transcription (By similarity). & Asymmetric dimethylation at Arg-18 by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 by PRMT5 is linked to gene repression (By similarity). & Methylation at Lys-5, Lys-37 and Lys-80 are linked to gene activation. Methylation at Lys-5 facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 and Lys-28 are linked to gene repression. Methylation at Lys-10 is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 and acetylation of H3 and H4. Methylation at Lys-5 and Lys-80 require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 and Lys-28 are enriched in inactive X chromosome chromatin (By similarity). & Phosphorylated at Thr-4 by GSG2/haspin during prophase and dephosphorylated during anaphase. At centromeres, specifically phosphorylated at Thr-12 from prophase to early anaphase. Phosphorylated at Ser-11 during the whole mitosis. Phosphorylation at Ser-11, which is linked to gene activation, prevents methylation at Lys-10 but facilitates acetylation of H3 and H4. Phosphorylated at Ser-29 by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation (By similarity). & Phosphorylation at 'Ser-11' is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at 'Ser-11' is important during interphase because it enables the transcription of genes following external stimulation, like stress or growth factors. Phosphorylation at 'Ser-11' is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylation at 'Ser-11' by AURKB/Aurora-B mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. & Ubiquitinated (By similarity). SIMILARITY: SwissProt: Q16695 ## Belongs to the histone H3 family.

产品使用声明
质量保证	Western Blot Analysis: 1:10,000 of this lot detected Dimethyl Histone H3 in HeLa acid extract.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at 2-8°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.