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Replacement Information Replacement Information 05-1117 is a recommended replacement for MAB3734 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB M Ascites Monoclonal Antibody 描述 产品目录编号 05-1117 Replaces MAB3734 描述 Anti-VEGF Antibody, clone VG1 Alternate Names - vascular endothelial growth factor isoform VEGF165
- Vascular permeability factor
- vascular endothelial growth factor
- vascular endothelial growth factor A
背景信息 VEGF (Vascular endothelial growth factor, VEGFA, VEGF-A), a dimeric ligand, is among the most potent angiogenic mitogens. VEGF is secreted by tumor cells and other cells exposed to hypoxia. VEGF is a highly specific mitogen for vascular endothelial cells. Seven VEGF isoforms (a, b, c, d, e, f, g) are generated as a result of alternative splicing from a single VEGF gene. The most commonly studied isoforms are VEGF121, VEGF165, and VEGF189 (f, d and b, respectively) All seven isoforms differ in their molecular mass and in biological properties such as their ability to bind to cell-surface heparan-sulfate proteoglycans. The expression of VEGF is potentiated and is secreted by tumor cells in response to hypoxia, by activated oncogenes, and by a variety of cytokines. 产品信息 格式 Ascites 控制 - NIH/3T3 cell lysate
演示 Unpurified mouse monoclonal IgG1κ in ascites fluid containing no preservatives. 应用 应用 Detect VEGF using this Anti-VEGF Antibody, clone VG1 validated for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Recombinant human VEGF 189aa isoform 克隆 VG1 宿主 Mouse 特异性 Detects the 121, 165, and 189 amino acid isoforms of VEGF. 同种型 IgG1κ 品种反应性 RatHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_033531.3
Entrez基因汇总 This gene product is a member of the PDGF/VEGF growth factor family. It is a mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis, endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Also, alternative translation initiation from non-AUG (CUG) and AUG start sites in some transcript variants, give rise to additional isoforms. 基因符号 - MVCD1
- MGC70609
- VPF
- VEGF-A
- VEGF
纯化方法 Unpurified UniProt编号 - P15692
UniProt汇总 FUNCTION: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Binds to the VEGFR1/Flt-1 and VEGFR2/Kdr receptors, heparan sulfate and heparin. Neuropilin-1 binds isoforms VEGF-165 and VEGF-145.
SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with PlGF (By similarity).
SUBCELLULAR LOCATION: Secreted. Note=VEGF121 is acidic and freely secreted. VEGF165 is more basic, has heparin-binding properties and, although a signicant proportion remains cell-associated, most is freely secreted. VEGF189 is very basic, it is cell-associated after secretion and is bound avidly by heparin and the extracellular matrix, although it may be released as a soluble form by heparin, heparinase or plasmin.
ALTERNATIVE PRODUCTS: 10 named isoforms [FASTA] produced by alternative splicing.产品使用声明 质量保证 Evaluated by Western Blot in NIH/3T3 cell lysate.
Western Blot Analysis: A 1:500 dilution of this antibody detected VEGF on 10 µg of NIH/3T3 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 12 months at -20°C from date of receipt.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,15-107,MAPK Pathway 1 Explorer Antibody MiniPack
下一件merck millipore产品：merck millipore,默克密理博,14-478,Yes Protein, active, 10 µg
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