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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1459 Replaces 04-1100 描述 Anti-SMAD1 Antibody, clone AS22 Alternate Names - Transforming growth factor-beta-signaling protein 1
- SMAD 1
- SMAD family member 1
- SMAD, mothers against DPP homolog 1
- MAD, mothers against decapentaplegic homolog 1
- Mad-related protein 1
- Mothers against DPP homolog 1
- Sma- and Mad-related protein 1
- TGF-beta signaling protein 1
- Sma- and Mad-related protein 1
- TGF-beta signaling protein 1
背景信息 Smad proteins are regulators of transcription which transduce signals from TGFβ Receptors. Smad proteins homotrimerize, and when activated, two distinct homotrimers assemble into a heterosextamer. Smad proteins fall into three classes. The receptor-regulated Smad proteins, Smad 1, 2, 3, 5, and 9 couple to specific receptors and are phosphorylated by those receptors. Phosphorylated receptor-regulated Smad proteins then bind to a co-Smad, such as Smad4/DPC4, and the complex moves to the nucleus where it associates with FAST-1 to stimulate target gene transcription. A third class of Smad proteins is the inhibitory group of Smad 6, 7, 8. Smad proteins have Mad-homology domains 1 and 2 (MH1 and MH2). MH1 domains are involved in DNA binding, while MH2 domains function in homotrimerization, receptor interaction and phosphorylation. The phosphorylation of Smad proteins is the regulatory signal in their activation, and can be monitored by the use of phosphorylation state-specific antibodies. 产品信息 格式 Purified 演示 Purified mouse monoclonal in 0.1M Tris-Glycine (pH7.4), 150mM NaCl with 0.05% NaN3. 应用 应用 Anti-SMAD1 Antibody, clone AS22 detects level of SMAD1 & has been published & validated for use in WB & IF. 主要应用 - Western Blotting
应用说明 Western Blot Analysis:
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.
生物信息 免疫原品种 GST-fusion protein corresponding to amino acids 1-110 of human SMAD1. 克隆 AS22 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Detects SMAD1 同种型 IgG1κ 品种反应性 MouseHuman Species Reactivity Note Human and Mouse. Other species were not tested. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001003688
Entrez基因汇总 The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signals of the bone morphogenetic proteins (BMPs), which are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. In response to BMP ligands, this protein can be phosphorylated and activated by the BMP receptor kinase. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq] 基因符号 - hSMAD1
- BSP-1
- BSP1
- CMT2D
- GARS
- HMN5
- JV4-1
- JV41
- MADH1
- MADR1
纯化方法 Protein G purfied UniProt编号 - Q15797
UniProt汇总 FUNCTION: Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD1 is a receptor-regulated SMAD (R-SMAD).
SUBUNIT: Interacts with HGS, NANOG and ZCCHC12 (By similarity). May form trimers with another SMAD1 and the co-SMAD SMAD4. Interacts with PEBP2-alpha subunit, CREB-binding protein (CBP), p300, SMURF1, SMURF2 and HOXC8. Associates with ZNF423 or ZNF521 in response to BMP2 leading to activate transcription of BMP target genes. Interacts with LBXCOR1.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4.
TISSUE SPECIFICITY: Ubiquitous. Highest expression seen in the heart and skeletal muscle.
PTM: Phosphorylated on serine by BMP type 1 receptor kinase.
PTM: Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.产品使用声明 质量保证 Routinely evaluated by Western Blot
Western Blot Analysis:
This lot detected SMAD1 at 1:500 dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
包装信息 数量 200 µg
上一件merck millipore产品：merck millipore,默克密理博,AB5454,Anti-TUC-4 Protein Antibody
下一件merck millipore产品：merck millipore,默克密理博,BSS-1005-B,Dulbecco's Phosphate Buffered Saline, 1X ES Cell Qualified
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