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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Fe, H, M, Mk, R WB, ICC, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB5454 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-TUC-4 Protein Antibody Alternate Names - Dihydropyrimidinase-like 3
- ULIP-1 Protein
- CRMP-4
- DRP-3
背景信息 TUC (TOAD [Turned On After Division]/ Ulip/CRMP) is a family of proteins that have emerged as strong candidates for a role in neuronal growth cone signaling. The TUC-4 family members reach their highest expression levels in early post-mitotic neurons during their peak periods of axonal growth. TUC-4 is not expressed in neural progenitors or adult neurons (with minor exception). Data suggests that TUC-4 plays a role in axonal outgrowth, pathfinding and possibly in the process that permits growing axons to choose proper routes and targets (Quinn, 1999). The TUC-4 protein is expressed primarily within the cytoplasm of postmitotic neurons as they begin their migration out of the ventricular zone into the developing cortical plate (Minturn, 1995a). TUC-4 is expressed in neuronal cell bodies, neurites, and along the most distal regions of neuronal growth cones, including the lamellipodia and filopodia (Minturn, 1995b). Expression of TUC-4 is observed by day E12 in rats coincident with the expression of the early neuronal marker class III beta-tubulin (Quinn, 1999). TUC-4 expression is rarely observed in neuronal cells expressing the mitotic marker PCNA (Minturn, 1995a). The expression of TUC-4 has been shown to be upregulated in response to NGF in rats (Minturn, 1995b) and in response to retinoic acid in a human neuroblastoma cell line (SMS-KCNR; Gaetano, 1997). 产品信息 格式 Serum 控制 - POSITIVE CONTROL: Fetal brain/spinal cord tissue. Human neuroblastoma cell line SMS-KCNR or rat PC12 cells.
演示 Rabbit Serum. Liquid containing 0.2% sodium azide. 应用 应用 Anti-TUC-4 Protein Antibody detects level of TUC-4 Protein & has been published & validated for use in IC, IH, IP & WB. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunohistochemistry: 1:1,000-1:5,000 using PFA (DAB detection).
Immunocytochemistry: 1:1,000-1:5,000 (DAB detection).
Western blot: 1:2,500-1:25,000 using alkaline phosphatase.
Immunoprecipitation
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Synthetic peptide corresponding to amino acids 499 to 511 of TUC-4a and amino acids 612-624 of TUC-4b (YDGPVFDLTTTPK) 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Specifically recognizes the TUC-4 (Ulip-1/CRMP-4/DRP-3) splice variants, TUC-4a (64 kDa) and the N-terminally extended TUC-4b (75 kDa) (Quinn, 2003). AB5454 has been reported to label early postmitotic neurons in prenatal rat brain. Labeling is observed primarily within the cytoplasm along the most distal regions of neuronal growth cones including lamellipodia and filopodia. The antibody is not reactive with the proteins TUC-1 (Ulip-3/CRMP-1), TUC-2 (Ulip-2/CRMP-2) or TUC-3 (Ulip-4/CRMP-3). It does not label neural progenitors or radial glia. 品种反应性 FelineHumanMouseMonkeyRat 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001387.2
基因符号 - DRP-3
- DPYSL3
- CRMP-4
- DRP3
- CRMP4
- ULIP
纯化方法 Unpurified UniProt编号 - Q14195
UniProt汇总 FUNCTION: SwissProt: Q14195 # May regulate vesicle function in the growth cone (By similarity).
SIZE: 570 amino acids; 61963 Da
SUBUNIT: Interacts with synaptic vesicle protein 2 and SH3A domain of intersectin (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, growth cone (By similarity). Note=Colocalizes with synaptic vesicle protein 2 in the central region of the growth cone (By similarity).
TISSUE SPECIFICITY: Mainly in heart and skeletal muscle. Also expressed strongly in fetal brain and spinal cord.
SIMILARITY: SwissProt: Q14195 ## Belongs to the dehydropyrimidinase family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µL
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下一件merck millipore产品：merck millipore,默克密理博,05-1459,Anti-SMAD1 Antibody, clone AS22
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