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merck millipore,默克密理博,05-1069,Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2
产品名称：Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2
产品型号：05-1069
This Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2 is validated for use in WB, IP, IC for the detection of phospho-PRAS40 (Thr246).
merck millipore,默克密理博,05-1069,Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1069 描述 Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2 Alternate Names - 40 kDa proline-rich AKT substrate <br />AKT1 substrate 1 (proline-rich) <br />proline-rich Akt substrate, 40 kDa
背景信息 PRAS40 (Proline Rich Akt Substrate, 40kDa), also known as AKT1S1, is a ubiquitously express protein that is phosphorylated on residue Thr246 via the PI3K/Akt pathway. It was originally discovered as an Akt substrate as it has the putative Akt phosphorylation motif. This phosphorylation allows it to bind 14-3-3 and Raptor of the mTOR complex mTORC1. PRAS40 is known to bind to and regulate mTORC1 (mTOR, Raptor, mLST8) kinases activity that is activated by insulin downstream of PI3K and Akt and subsequently phosphorylates p70S6K and 4EBP1. PRAS40 is known to have a putative TOS motif (FVMDE) that allows it bind to Raptor of mTORC1. It is thought that through its binding to the Raptor, PRAS40 inhibits the kinase activity of mTORC1 by preventing to bind to its substrates such as p70S6K and 4EBP1. 产品信息 格式 Purified 演示 Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 This Anti-phospho-PRAS40 (Thr246) Antibody, clone 4H2.2 is validated for use in WB, IP, IC for the detection of phospho-PRAS40 (Thr246). 主要应用 - Immunocytochemistry
- Western Blotting
生物信息 免疫原品种 Synthetic peptide corresponding to the amino acids surrounding and including phosphorylated Thr246 of human PRAS40. 表位 Phosphorylated Thr246 克隆 4H2.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Detects PRAS40 when phosphorylated on Thr246. 同种型 IgG1κ 品种反应性 MouseHuman Species Reactivity Note Human and Mouse. Predicted to cross-react with rat and bovine based sequence homology of immunizing peptide. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001092102.1
Entrez基因汇总 AKT1S1 is a proline-rich substrate of AKT (MIM 164730) that binds 14-3-3 protein (see YWHAH, MIM 113508) when phosphorylated (Kovacina et al., 2003 [PubMed 12524439]).[supplied by OMIM] 基因符号 - Lobe
- PRAS40
- AKT1S1
修改 - Phosphorylation
纯化方法 Protein G purfied UniProt编号 - Q96B36
UniProt汇总 FUNCTION: May play an important role in phosphatidylinositol 3-kinase (PI3K)-AKT1 survival signaling. Substrate for AKT1 phosphorylation, but can also be activated by AKT1-independent mechanisms. Its role in survival signaling pathways may be modulated by oxidative stress. May also play a role in nerve growth factor-mediated neuroprotection.
SUBUNIT STRUCTURE: The phosphorylated form interacts with 14-3-3.
SUBCELLULAR LOCATION: Cytoplasm - cytosol. Note= Found in the cytosolic fraction of the brain.
TISUE SPECIFICITY: Widely expressed with highest levels of expression in liver and heart. Expressed at higher levels in cancer cell lines (e.g. A549 and HeLa) than in normal cell lines (e.g. HEK293).
产品使用声明 质量保证 Evaluated by western blot on untreated and PDGF-treated NIH/3T3 cell lysate.
Western Blot Analysis:
1:1,000 dilution of this antibody was used to detect PRAS40 in PDGF-treated NIH/3T3 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8ºC from date of receipt. 包装信息 数量 100 µg
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