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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Purified Monoclonal Antibody 描述 产品目录编号 04-816 Replaces 05-816 描述 Anti-phospho-PPARγ (Ser82) Antibody, clone AW504 | 04-816 Alternate Names - peroxisome proliferator-activated receptor gamma 1
- Nuclear receptor subfamily 1 group C member 3
- PPAR gamma
- peroxisome proliferative activated receptor gamma
- peroxisome proliferator-activated receptor gamma
背景信息 Peroxisome proliferator-activated receptors (PPARs) are nuclear receptors involved in lipid transport and metabolism. As such, their roles in chronic diseases such as diabetes, obesity, atherosclerosis and cancer are heavily investigated. Transcriptional activity of PPARs is regulated by fatty acid binding. Three PPAR isotypes have been identified: , and . PPAR stimulates lipolysis of circulating triglycerides and the subsequent uptake of fatty acids into adipose cells. PPARs can bind to DNA only as a heterodimer with the retinoid X receptor (RXR). 产品信息 格式 Purified 控制 - 3T3/L1 cells differentiated into adipocytes, PMA-stimulated THP-1 cells.
演示 Cultured supernantant containing 0.05% sodium azide. 应用 主要应用 - Western Blotting
生物信息 免疫原品种 KLH-conjugated, synthetic peptide containing the sequence PApSPP in which pS corresponds to phosphoserine at residue 82 of human PPARγ. 表位 pSer82 克隆 AW504 浓缩 Cultured supernantant containing 0.05% sodium azide. 宿主 Rabbit 特异性 PPARγ phosphorylated at Ser82, Mr 55 & 60 kDa. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Human and mouse. Predicted to cross-react with rat based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005028.4
Entrez基因汇总 This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) subfamily of nuclear receptors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes. Three subtypes of PPARs are known: PPAR-alpha, PPAR-delta, and PPAR-gamma. The protein encoded by this gene is PPAR-gamma and is a regulator of adipocyte differentiation. Additionally, PPAR-gamma has been implicated in the pathology of numerous diseases including obesity, diabetes, atherosclerosis and cancer. Alternatively spliced transcript variants that encode different isoforms have been described. 基因符号 - PPARG2
- CIMT1
- NR1C3
- OTTHUMP00000185030
- OTTHUMP00000185033
- OTTHUMP00000185037
- PPAR-gamma
- PPARG1
修改 - Phosphorylation
UniProt编号 - P37231
UniProt汇总 FUNCTION:Receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. Once activated by a ligand, the receptor binds to a promoter element in the gene for acyl-CoA oxidase and activates its transcription. It therefore controls the peroxisomal beta-oxidation pathway of fatty acids. Key regulator of adipocyte differentiation and glucose homeostasis.
SUBUNIT STRUCTURE: Forms a heterodimer with the retinoic acid receptor RXRA called adipocyte-specific transcription factor ARF6. Interacts with NCOA6 coactivator, leading to a strong increase in transcription of target genes. Interacts with coactivator PPARBP, leading to a mild increase in transcription of target genes. Interacts with FAM120B. Interacts with PRDM16 By similarity. Interacts with NOCA7 in a ligand-inducible manner. Interacts with NCOA1 LXXLL motifs. Interacts with TGFB1I1. Interacts with DNTTIP2.
SUBCELLULAR LOCATION:Nucleus.
TISSUE SPECIFICITY:Highest expression in adipose tissue. Lower in skeletal muscle, spleen, heart and liver. Also detectable in placenta, lung and ovary.
POLYMORPHISM: Genetic variation in PPARG may influence body mass index (BMI) [MIM:606641]. BMI reflects the amount of fat, lean mass, and body build.
INVOLVEMENT IN DISEASE: Defects in PPARG can lead to type 2 insulin-resistant diabetes and hyptertension.Defects in PPARG may be associated with susceptibility to obesity [MIM:601665].Defects in PPARG may be associated with colon cancer. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) [MIM:604367]. Familial partial lipodystrophies (FPLD) are a heterogeneous group of genetic disorders characterized by marked loss of subcutaneous (sc) fat from the extremities. Affected individuals show an increased preponderance of insulin resistance, diabetes mellitus and dyslipidemia. Variation in PPARG is associated with carotid intimal medial thickness 1 (CIMT1) [MIM:609338]. CIMT is a measure of atherosclerosis that is independently associated with traditional atherosclerotic cardiovascular disease risk factors and coronary atherosclerotic burden. 35 to 45% of the variability in multivariable-adjusted CIMT is explained by genetic factors.
SEQUENCE SIMILARITIES: Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain.产品使用声明 质量保证 Evaluated by western blot on NIH/3T3 L1 cells that were undifferentiated and differentiated into adipocytes.
Western Blot Analysis: 1:500 dilution of this antibody detected PPARγ phosphorylated at Ser82 in undifferentiated and acipocyte differentiated NIH/3T3 L1 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µl
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