merck millipore,默克密理博,04-440,Anti-Caspase-3 (Pro) Antibody, rabbit monoclonal

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merck millipore,默克密理博,04-440,Anti-Caspase-3 (Pro) Antibody, rabbit monoclonal
产品名称：Anti-Caspase-3 (Pro) Antibody, rabbit monoclonal
产品型号：04-440
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Caspase-3 (Pro) Antibody, Rabbit is valida More>>

merck millipore,默克密理博,04-440,Anti-Caspase-3 (Pro) Antibody, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-440,Anti-Caspase-3 (Pro) Antibody, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	WB, FC	Rb	Purified	Monoclonal Antibody

描述
产品目录编号	04-440
Replaces	04-1088
品牌系列	Upstate
商名	Upstate
描述	Anti-Caspase-3 (Pro) Antibody, rabbit monoclonal

产品信息
格式	Purified
演示	In 60% storage buffer (50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 0.01% sodium azide and 0.05% BSA) and 40% glycerol.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-Caspase-3 (Pro) Antibody, Rabbit is validated for use in FC, IF, IP, WB, IH(P) for the detection of Caspase-3 (Pro).
主要应用	Western Blotting Flow Cytometry

生物信息
免疫原品种	KLH-conjugated synthetic peptide composed of residues at the N-terminus of human Pro-Caspase-3.
宿主	Rabbit
特异性	Recognizes a region composed of residues at the N-terminus of human Pro-Caspase-3. This antibody will only detect the pro-form of Caspase-3
同种型	IgG
品种反应性	MouseHuman
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_032991.2 NM_004346.3
Entrez基因汇总	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein.
基因符号	Yama CASP3 apopain CASP-3 procaspase3 CPP32B SCA-1 CPP-32 Apopain CPP32
UniProt编号	P42574
UniProt汇总	FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. SIZE: 277 amino acids; 31608 Da SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.

产品使用声明
质量保证	Routinely evaluated by immunoblot.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

储存和货运信息

存储条件

2 year at -20°C from date of shipment. Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.