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Replacement Information Replacement Information This antibody is a recommended replacement for CBL520. 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H IHC, FC, WB M Purified Monoclonal Antibody 描述 产品目录编号 04-1528 Replaces CBL520 描述 Anti-CD86 Antibody, clone IT2.2 Alternate Names - CTLA-4 counter-receptor B7.2
- Activation B7-2 antigen
- B-lymphocyte activation antigen B7-2
- B7-2 antigen
- CD28 antigen ligand 2
- CD86 antigen
- CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
- CD86 molecule
背景信息 CD86 (T-lymphocyte activation antigen CD86, activation B7-2 antigen or CTLA-4 counter-receptor B7.2) is a molecule expressed on antigen-presenting cells that provide costimulatory signals necessary for T cell activation and survival. CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4 (cytotoxic T-lymphocyte-associated protein 4). Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. Along with CD80, CD86 provides stimuli to prime T cells against antigens presented by antigen-presenting cells. CD86 is expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, macrophages, dendritic cells, and Langerhans cells, plus astrocytes. CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T and B cell-specific stimuli with a peak expression after 18-42 hours of culture. CD86 has also been shown to be involved in immunoglobulin class switching and triggering of mouse NK cell-mediated cytotoxicity. 产品信息 格式 Purified 控制 - Human PBMC's
演示 Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-CD86 Antibody, clone IT2.2 detects level of CD86 & has been published & validated for use in FC, WB, IH. 主要应用 - Immunohistochemistry
- Flow Cytometry
- Western Blotting
应用说明 Western Blot Analysis: A previous lot was used by an independent laboratory in WB (Esser, M.T., et al., 2001).
Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Geissmann, F., et al., 2001).生物信息 免疫原品种 Human LCL whole cells. 表位 Unknown 克隆 IT2.2 宿主 Mouse 特异性 This antibody recognizes CD86. 同种型 IgG2bκ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_008820
Entrez基因汇总 This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined. [provided by RefSeq]. 基因符号 - LAB72
- CD86
- B7-2
- B70
- BU63
- CD28LG2
- FUN-1
纯化方法 Protein G purfied UniProt编号 - P42081
UniProt汇总 FUNCTION: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation.
SUBUNIT STRUCTURE: Interacts with MARCH8. Interacts with human herpesvirus 8 MIR2 protein. Interacts with adenovirus subgroup B fiber proteins and acts as a receptor for these viruses.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Expressed by activated B-lymphocytes and monocytes.
PTM: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
SEQUENCE SIMILARITIES: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.产品使用声明 质量保证 Evaluated by Flow Cytometry in human PBMC's.
Flow Cytometry Analysis: A 1:100-125 dilution of this antibody detected CD86 in human PBMC's.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 50 µL
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