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merck millipore,默克密理博,04-1462,Anti-hnRNP F Antibody, clone 3H4
产品名称：Anti-hnRNP F Antibody, clone 3H4
产品型号：04-1462
Use Anti-hnRNP F Antibody, clone 3H4 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect hnRNP F also known as heterogeneous nuclear ribonucleoprotein F.
merck millipore,默克密理博,04-1462,Anti-hnRNP F Antibody, clone 3H4
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB M Purified Monoclonal Antibody 描述 产品目录编号 04-1462 描述 Anti-hnRNP F Antibody, clone 3H4 Alternate Names - mcs94-1
- heterogeneous nuclear ribonucleoprotein F
- nucleolin-like protein
- HnRNP F protein
- Nucleolin-like protein mcs94-1
背景信息 The hnRNP F protein is member of a subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs).
The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and associate with pre-mRNAs in the nucleus. These complexes are associated with pre-mRNA processing and other aspects of mRNA metabolism and transport. There are more than 20 different heterogeneous nuclear ribonucleoproteins (hnRNPs) associated with pre-mRNAs in the nucleus of mammalian cells. These different hnRNPs display a range of affinities for a variety of ribonucleotidehomopolymers and ssDNA sequences. In constrast, hnRNP F protein as well as hnRNP H (a highly related protein) appear to bind only to poly(rG). These unique RNA-binding properties, as well as the amino acid sequence and other data showing a distinct intranuclear localization of hnRNP F and hnRNP H suggest they are unique from other hnRNP and play an important role in the processing of RNAs containing guanosine-rich sequences.产品信息 格式 Purified 控制 - K562 cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-hnRNP F Antibody, clone 3H4 (Mouse Monoclonal Antibody) validated in WB, IP, ICC to detect hnRNP F also known as heterogeneous nuclear ribonucleoprotein F. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunoprecipitation Analysis:
A representative lot was used by an independent laboratory for IP. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)
Immunofluorescence Analysis:
A representative lot was used by an independent laboratory in IF. (Matunis, M.J., et al. (1994). Nucleic Acids Res. 22(6):1059–1067.)生物信息 免疫原品种 Recombinant protein corresponding to human hnRNP F. 表位 Unknown 克隆 clone 3H4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes hnRNP F. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001091674
Entrez基因汇总 This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq]. 基因符号 - hnRNP F
- HNRPF
纯化方法 Protein G purfied UniProt编号 - P52597
UniProt汇总 FUNCTION: Component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Probably binds G-rich sequences in pre-mRNAs.
SUBUNIT STRUCTURE: Identified in the spliceosome C complex, at least composed of AQR, ASCC3L1, C19orf29, CDC40, CDC5L, CRNKL1, DDX23, DDX41, DDX48, DDX5, DGCR14, DHX35, DHX38, DHX8, EFTUD2, FRG1, GPATC1, HNRPA1, HNRPA2B1, HNRPA3, HNRPC, HNRNPF, HNRPH1, HNRPK, HNRPM, HNRPR, HNRPU, KIAA1160, KIAA1604, LSM2, LSM3, MAGOH, MORG1, PABPC1, PLRG1, PNN, PPIE, PPIL1, PPIL3, PPWD1, PRPF19, PRPF4B, PRPF6, PRPF8, RALY, RBM22, RBM8A, RBMX, SART1, SF3A1, SF3A2, SF3A3, SF3B1, SF3B2, SF3B3, SFRS1, SKIV2L2, SNRPA1, SNRPB, SNRPB2, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF, SNRPG, SNW1, SRRM1, SRRM2, SYF2, SYNCRIP, TFIP11, THOC4, U2AF1, WDR57, XAB2 and ZCCHC8. Interacts with TBP and TXNL4/DIM1.
SUBCELLULAR LOCATION: Nucleus › nucleoplasm.
TISSUE SPECIFICITY: Expressed ubiquitously.
DOMAIN: The N-terminal RRM domains are responsible for recognizing the G-tract of BCL-X RNA.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated.
SEQUENCE SIMILARITIES: Contains 3 RRM (RNA recognition motif) domains.产品使用声明 质量保证 Evaluated by Western Blot in K562 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected hnRNP F on 10 µg of K562 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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