merck millipore,默克密理博,04-1049,Anti-β-Tubulin Antibody, class III (CT), clone EP1569Y, rabbit monoclonal

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merck millipore,默克密理博,04-1049,Anti-β-Tubulin Antibody, class III (CT), clone EP1569Y, rabbit monoclonal
产品名称：Anti-β-Tubulin Antibody, class III (CT), clone EP1569Y, rabbit monoclonal
产品型号：04-1049
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect β-Tubulin using this Anti-β-Tubulin Antibody, More>>

merck millipore,默克密理博,04-1049,Anti-β-Tubulin Antibody, class III (CT), clone EP1569Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1049,Anti-β-Tubulin Antibody, class III (CT), clone EP1569Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	FC, WB, ICC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1049
描述	Anti-β-Tubulin Antibody, class III (CT), clone EP1569Y, rabbit monoclonal
Alternate Names	tubulin-specific chaperone d Beta-tubulin cofactor D Tubulin-folding cofactor D tubulin folding cofactor D
背景信息	Microtubules are constituent parts of the mitotic apparatus, cilia, flagella, and elements of the cytoskeleton. They consist principally of 2 soluble proteins, alpha- and beta-tubulin, each of about 55,000 Da. Antibodies against beta Tubulin are useful as loading controls for Western Blotting. However it should be noted that levels of beta Tubulin may not be stable in certain cells. For example, expression of tubulin in adipose tissue is very low (Spiegelman and Farmer, Cell, 1982, 29(1):53-60) and therefore beta Tubulin should not be used as loading control for these tissues.

产品信息
格式	Unpurified
控制	HeLa cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.18 M NaCl containing 40% Glycerol, 0.25% sodium azide, 0.25 mM EDTA, 25 mM Citric Acid, and 0.5% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect β-Tubulin using this Anti-β-Tubulin Antibody, class III (C-term), clone EP1569Y, Rabbit validated for use in WB, IC, FC, IP, IH(P).
主要应用	Flow Cytometry Western Blotting Immunocytochemistry
应用说明	Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected β-Tubulin in human breast carcinoma tissue. Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC. Flow Cytometry: A 1:10 dilution of a previous lot was used in flow cytometry. Immunoprecipitation Analysis: A 1:40 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to the C-terminus of human class III β-Tubulin.
表位	C-terminus
克隆	EP1569Y
宿主	Rabbit
特异性	This antibody recognizes β-Tubulin at and around the C-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse, and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_006077.2
基因符号	tfcD KIAA0988 SSD-1 SSD1
纯化方法	Unpurified
UniProt编号	Q9BTW9
UniProt汇总	FUNCTION: Tubulin-folding protein; involved in the first step of the tubulin folding pathway. Modulates microtubule dynamics by capturing GTP-bound beta-tubulin (TUBB). SUBUNIT STRUCTURE: Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. TISSUE SPECIFICITY: Ubiquitously expressed. INDUCTION: Down-regulated by shear stress. SEQUENCE SIMILAIRITES: Belongs to the TBCD family. Contains 3 HEAT repeats.

产品使用声明
质量保证	Evaluated by Western Blot on HeLa cell lysates. Western Blot Analysis: 1:10,000 dilution of this antibody was used to detect β-Tubulin in HeLa cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.