- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, M IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB5820-50UL 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Syntaxin 1 Antibody 产品信息 格式 Affinity Purified 控制 - CONTROL ANTIGEN: Included free of charge with the antibody is 50 μg of control antigen (lyophilized powder). The stock solution of the antigen can be made up using 100 mL of PBS. For positive control, in Western blot using 20 ng of protein per Minigel lane. For negative control, preincubate 5 μg of fusion protein with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
演示 Affinity purified immunoglobulin. Lyophilized from phosphate buffered saline, pH 7.4, containing 1% BSA, 5% sucrose as a stabilizer and 0.025% sodium azide as a preservative. Reconstitute with 50 μL of sterile deionized water. Centrifuge antibody preparation before use (10,000 xg for 5 min). 应用 应用 This Anti-Syntaxin 1 Antibody is validated for use in WB, IH for the detection of Syntaxin 1. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western blot: 1:700-1:1,250 (0.8-1.5 g/mL) using ECL on rat brain membranes.
Immunohistochemistry: AB5820 is directed against an intracellular epitope. Thus, a procedure including permeabilization of cells with 0.2% Triton X 100 is recommended. It is recommended that you start with a working concentration of 30 μg/mL (1:33).
Dilutions should be made using a carrier protein such as BSA (1-3%)
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 GST fusion protein corresponding to a cytoplasmic part of rat Syntaxin 1A (Accession number P32851) (Bennet et al. 1992; Inoue & Akagawa 1992). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Syntaxin 1A and Syntaxin 1B. The epitope does not share homology with any other known proteins. 品种反应性 RatMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_004603.2
Entrez基因汇总 Synaptic vesicles store neurotransmitters that are released during calcium-regulated exocytosis. The specificity of neurotransmitter release requires the localization of both synaptic vesicles and calcium channels to the presynaptic active zone. Syntaxins function in this vesicle fusion process. Syntaxins also serve as a substrate for botulinum neurotoxin type C, a metalloprotease that blocks exocytosis and has high affinity for a molecular complex that includes the alpha-latrotoxin receptor (MIM 600565) which produces explosive exocytosis (Zhang et al., 1995 [PubMed 7622072]).[supplied by OMIM] 基因符号 - p35-1
- STX1A
- HPC-1
- STX1
- Syntaxin-1A
UniProt编号 - Q16623
UniProt汇总 FUNCTION: SwissProt: Q16623 # Potentially involved in docking of synaptic vesicles at presynaptic active zones. May play a critical role in neurotransmitter exocytosis.
SIZE: 288 amino acids; 33023 Da
SUBUNIT: Part of the SNARE core complex containing SNAP25, VAMP2 and STX1A. This complex binds to CPLX1. Binds SYTL4 and STXBP6. Found in a ternary complex with STX1A and SNAP25 (By similarity). Found in a complex with VAMP8 and SNAP23. Interacts with VAPA and SYBU.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type IV membrane protein. & Isoform 2: Secreted (Probable).
TISSUE SPECIFICITY: Isoform 1 is highly expressed in embryonic spinal chord and ganglia and in adult cerebellum and cerebral cortex. Isoform 2 is expressed in heart, liver, fat, skeletal muscle, kidney and brain.
DISEASE: SwissProt: Q16623 # Haploinsufficiency of STX1A may be the cause of certain cardiovascular and musculo-skeletal abnormalities observed in Williams-Beuren syndrome (WBS), a rare developmental disorder. It is a contiguous gene deletion syndrome involving genes from chromosome band 7q11.23.
SIMILARITY: SwissProt: Q16623 ## Belongs to the syntaxin family. & Contains 1 t-SNARE coiled-coil homology domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After reconstitution maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µL
上一件merck millipore产品：merck millipore,默克密理博,SF-429,miR-215; Human, Cyanine 5, RNA Detection Probe,SmartFlare
下一件merck millipore产品：merck millipore,默克密理博,FCMAB271A6,Milli-Mark™ Anti-phospho-ATF2 (Thr69/71) Antibody, Alexa Fluor 647, clone AW65
相关产品
merck millipore,默克密理博,ABE1000,Anti-FOXM1 Antibody
merck millipore,默克密理博,69036,Factor Xa, Restriction Grade, Bovine Plasma
merck millipore,默克密理博,AB1276,Anti-Cytochrome P450 Enzyme CYP3A2 Antibody
merck millipore,默克密理博,SF-429,miR-215; Human, Cyanine 5, RNA Detection Probe,SmartFlare
merck millipore,默克密理博,354400,Glutaraldehyde, 25% Aqueous Solution
merck millipore,默克密理博,06-1073,Anti-IL-20R alpha Antibody
merck millipore,默克密理博,AB740-K,Anti-Apolipoprotein A-1, Gt Anti-Hu