merck millipore,默克密理博,04-1041,Anti-Musashi-1 (NT) Antibody, clone EP1302, rabbit monoclonal

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merck millipore,默克密理博,04-1041,Anti-Musashi-1 (NT) Antibody, clone EP1302, rabbit monoclonal
产品名称：Anti-Musashi-1 (NT) Antibody, clone EP1302, rabbit monoclonal
产品型号：04-1041
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Musashi-1 (N-term) Antibody, clone EP1302, Rabb More>>

merck millipore,默克密理博,04-1041,Anti-Musashi-1 (NT) Antibody, clone EP1302, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1041,Anti-Musashi-1 (NT) Antibody, clone EP1302, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	ICC, WB, FC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1041
描述	Anti-Musashi-1 (NT) Antibody, clone EP1302, rabbit monoclonal
Alternate Names	musashi homolog 1 (Drosophila) Musashi (Drosophila) homolog 1 musashi 1
背景信息	In mammals, the Musashi family is important for cell fate determination, playing roles in maintenance of the stem-cell state, differentiation and tumorigenesis. Musashi-1 (also known as Msi1), is selectivley expressed in neural progenitor cells, including neural stem cells. Outside the nervous system, Musashi-1 is a selective marker for intestinal stem or early lineage cells. Musashi-1 interacts with the Notch pathway during asymmetric cell division by binding the 3' UTR of Numb. Numb is prevented from repressing Notch signaling when Musashi-1 is present.

产品信息
格式	Unpurified
控制	SHSY5Y cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Musashi-1 (N-term) Antibody, clone EP1302, Rabbit is an antibody against Musashi-1 (N-term) for use in WB, IH(P), FC, IC & IP.
主要应用	Immunocytochemistry Western Blotting Flow Cytometry
应用说明	Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected Musashi-1 in human brain tissue. Immunocytochemistry Analysis: A 1:250 - 500 dilution from a previous lot detected Musashi-1 in PC12 cells. Flow Cytometry: A 1:40 dilution of a previous lot was used in flow cytometry. Immunoprecipitation Analysis: A 1:50 dilution from a previous lot was used in IP.

生物信息
免疫原品种	Synthetic peptide corresponding to residues on the N-terminus of human Musashi-1.
表位	N-terminus
克隆	EP1302
宿主	Rabbit
特异性	This antibody recognizes Musashi-1 at and around the N-terminus.
同种型	IgG
品种反应性	RatHuman
Species Reactivity Note	Human and rat.
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002433.1
Entrez基因汇总	This gene encodes a protein containing two conserved tandem RNA recognition motifs. Similar proteins in other species function as RNA-binding proteins and play central roles in posttranscriptional gene regulation. Expression of this gene has been correlated with the grade of the malignancy and proliferative activity in gliomas and melanomas. A pseudogene for this gene is located on chromosome 11q13.
基因符号	Musashi-1
纯化方法	Unpurified
UniProt编号	O43347
UniProt汇总	FUNCTION: RNA binding protein that regulates the expression of target mRNAs at the translation level. Regulates expression of the NOTCH1 antagonist NUMB. Binds RNA containing the sequence 5'-GUUAGUUAGUUAGUU-3' and other sequences containing the pattern 5'-[GA]U1-3AGU-3'. May play a role in the proliferation and maintenance of stem cells in the central nervous system By similarity. SUBCELLULAR LOCATION: Cytoplasm By similarity. Nucleus By similarity. TISSUE SPECIFICITY: Detected in fetal kidney, brain, liver and lung, and in adult brain and pancreas. Detected in hepatoma cell lines. DOMAIN: The first RNA recognition motif binds more strongly to RNA compared to the second one By similarity. SEQUENCE SIMILARITIES: Belongs to the Musashi family. Contains 2 RRM (RNA recognition motif) domains.

产品使用声明
质量保证	Evaluated by Western Blot on SHSY5Y cell lysates. Western Blot Analysis: 1:2,000 dilution of this antibody was used to detect Musashi-1 in SHSY5Y cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.