merck millipore,默克密理博,04-1159,Anti-LEF-1/TCF-1 α Antibody, clone EPR2029Y, rabbit monoclonal

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merck millipore,默克密理博,04-1159,Anti-LEF-1/TCF-1 α Antibody, clone EPR2029Y, rabbit monoclonal
产品名称：Anti-LEF-1/TCF-1 α Antibody, clone EPR2029Y, rabbit monoclonal
产品型号：04-1159
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1159,Anti-LEF-1/TCF-1 α Antibody, clone EPR2029Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1159,Anti-LEF-1/TCF-1 α Antibody, clone EPR2029Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1159
描述	Anti-LEF-1/TCF-1 α Antibody, clone EPR2029Y, rabbit monoclonal
Alternate Names	lymphoid enhancer-binding factor 1 T cell-specific transcription factor 1-alpha lymphoid enhancer binding factor-1
背景信息	Lymphoid Enhancer Factor-1 (LEF-1) is an HMG 1/2-like DNA binding/bending protein and is a member of the LEF/TCF transcription factor family. There are four LEF/TCF family members in mammalian systems (LEF-1, TCF-1, TCF-3 and TCF-4), and orthologs to these factors have been identified in many different species. LEF/TCFs are downstream mediators of Wnt/Wingless signals. Wnt signaling drives cell polarity, cell fate and cell growth decisions in embryonic tissues and in post-natal tissues that continue to develop from mitotically active stem cell precursors. Misregulation of Wnt signaling is also implicated as a root cause of many different cancers, such as colon cancer, melanoma, breast cancer, prostate cancer and others.

产品信息
格式	Unpurified
控制	Jurkat cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry (Paraffin) Analysis: A 1:100-250 dilution from a previous lot detected LEF-1/TCF-1 α in human tonsil tissue. Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.

生物信息
免疫原品种	Synthetic peptide corresponding to residues in human LEF-1/TCF-1 α.
表位	a.a. 236-242
克隆	EPR2029Y
宿主	Rabbit
特异性	This antibody recognizes an epitope in amino acids 236-242 of LEF-1. This region is found within the transactivation domain of LEF-1. However, it is not present in some splice variants.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NM_016269.2
基因符号	TCF1ALPHA DKFZp586H0919 LEF-1 TCF1-alpha
纯化方法	Unpurified
UniProt编号	Q9UJU2
UniProt汇总	FUNCTION: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 By similarity. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. SUBUNIT STRUCTURE: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG By similarity. Binds THOC4, MDFI and MDFIC. SUBCELLULAR LOCATION: Nucleus By similarity. Note: Found in nuclear bodies upon PIASG binding By similarity. TISSUE SPECIFICITY: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. DOMAIN: Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors. SEQUENCE SIMILARITIES: Belongs to the TCF/LEF family. Contains 1 HMG box DNA-binding domain.

产品使用声明
质量保证	Evaluated by Western Blot on Jurkat cell lysates. Western Blot Analysis: A 1:10,000-20,000 dilution of this antibody was used to detect LEF-1/TCF-1 α in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

包装信息
数量	100 µL

merck millipore,默克密理博,04-1159,Anti-LEF-1/TCF-1 α Antibody, clone EPR2029Y, rabbit monoclonal

上一件merck millipore产品：merck millipore,默克密理博,07-718,Anti-Histone H2A.Z Antibody
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