merck millipore,默克密理博,04-1009,Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal

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merck millipore,默克密理博,04-1009,Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal
产品名称：Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal
产品型号：04-1009
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1009,Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1009,Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1009
描述	Anti-phospho-acetyl CoA Carboxylase 1 (ACC1) (Ser79) Antibody, clone EP1885Y, rabbit monoclonal
Alternate Names	acetyl-Coenzyme A carboxylase alpha acetyl-CoA carboxylase 1 acetyl-CoA carboxylase-alpha
背景信息	Acetyl-CoA carboxylase (ACC) is a biotin-dependent enzyme that catalyzes carboxylation of acetyl-CoA to produce malonyl-CoA through its two catalytic activities, biotin carboxylase (BC) and carboxyltransferase (CT). ACC is a multi-subunit enzyme in most prokaryotes, whereas it is a large, multi-domain enzyme in most eukaryotes. The activity of ACC can be controlled at the transcriptional level as well as by small molecule modulators and covalent modification. The human genome contains the genes for two different ACCs - ACACA and ACACB. The activity of the enzyme is controlled by reversible phosphorylation. The activities of the enzyme is inhibited if phosphorylated; the phosphorylation takes place when the hormones glucagon or epinephrine bind to the receptors or the energy status of the cell is low, leading to the activation of the AMP-activated protein kinase. The presence of fatty acid inhibits the activities of the enzyme. When insulin binds to its receptors, it activates a phosphatase to dephosphorylate the enzyme; the activities of the acetyl-CoA carboxylase is thus enhanced. Acetyl-CoA carboxylase has recently become a target in the design of new anti-obesity and antibiotic drugs.

产品信息
格式	Unpurified
控制	Untreated and LP-treated A431 cell lysate
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunocytochemistry Analysis: A 1:100-250 dilution from a previous lot was used in IC.

生物信息
免疫原品种	Phospho-specific peptide corresponding to residues surrounding Ser79 of human ACC1.
表位	Phosphorylated Ser79
克隆	EP1885Y
宿主	Rabbit
特异性	This antibody detects ACC1 phosphorylated at Ser79.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_942131.1
Entrez基因汇总	Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriche in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.
基因符号	OTTHUMP00000164069 ACAC ACC ACC-alpha ACC1 ACCA EC 6.4.1.2
修改	Acetylation Phosphorylation
纯化方法	Unpurified
UniProt编号	Q13085
UniProt汇总	FUNCTION: Catalyzes the rate-limiting reaction in the biogenesis of long-chain fatty acids. Carries out three functions: biotin carboxyl carrier protein, biotin carboxylase and carboxyltransferase. CATALYTIC ACTIVITY: ATP + acetyl-CoA + HCO3- = ADP + phosphate + malonyl-CoA. ATP + biotin-carboxyl-carrier protein + CO2 = ADP + phosphate + carboxybiotin-carboxyl-carrier protein. COFACTOR: Biotin. Binds 2 manganese ions per subunit. ENZYME REGULATION: By phosphorylation By similarity. PATHWAY: Lipid metabolism; malonyl-CoA biosynthesis; malonyl-CoA from acetyl-CoA: step 1/1. SUBUNIT STRUCTURE: Interacts in its inactive phosphorylated form with the BRCT domains of BRCA1 which prevents ACACA dephosphorylation and inhibits lipid synthesis. SUBCELLULAR LOCATION: Cytoplasm. TISSUE SPECIFICITY: Expressed in brain, placental, skeletal muscle, renal, pancreatic and adipose tissues; expressed at low level in pulmonary tissue; not detected in the liver. PTM: Phosphorylation on Ser-1263 is required for interaction with BRCA1. INVOLVEMENT IN DISEASE: Defects in ACACA are a cause of ACACA deficiency [MIM:200350]; also called ACAC or ACC deficiency. ACACA deficiency is an inborn error of de novo fatty acid synthesis. The disorder is associated with severe brain damage, persistent myopathy and poor growth. SEQUENCE SIMILARITIES: Contains 1 ATP-grasp domain. Contains 1 biotin carboxylation domain. Contains 1 biotinyl-binding domain. Contains 1 carboxyltransferase domain.

产品使用声明
质量保证	Evaluated by Western Blot in untreated and LP-treated A431 cell lysates. Western Blot Analysis: A 1:5,000-10,000 dilution of this antibody was used to detect ACC1 in untreated and LP-treated A431 cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.