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品种反应性 主要应用 宿主 格式 抗体类型 M IHC, FC R Purified Monoclonal Antibody 描述 产品目录编号 04-1527 描述 Anti-CD86 Antibody, clone PO.3 Alternate Names - CTLA-4 counter-receptor B7.2
- Activation B7-2 antigen
- B-lymphocyte activation antigen B7-2
- B7-2 antigen
- CD28 antigen ligand 2
- CD86 antigen
- CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)
- CD86 molecule
背景信息 CD86 (T-lymphocyte activation antigen CD86, activation B7-2 antigen or CTLA-4 counter-receptor B7.2) is a molecule expressed on antigen-presenting cells that provide costimulatory signals necessary for T cell activation and survival. CD86 is the ligand for two proteins at the cell surface of T cells, CD28 antigen and CTLA-4 (cytotoxic T-lymphocyte-associated protein 4). Binding of CD86 with CTLA-4 negatively regulates T-cell activation and diminishes the immune response. Along with CD80, CD86 provides stimuli to prime T cells against antigens presented by antigen-presenting cells. CD86 is expressed on a broad spectrum of leukocytes, including B lymphocytes, T lymphocytes, macrophages, dendritic cells, and Langerhans cells, plus astrocytes. CD86 is expressed at low levels by freshly explanted peripheral B and T cells, and its expression is substantially increased by a variety of T and B cell-specific stimuli with a peak expression after 18-42 hours of culture. CD86 has also been shown to be involved in immunoglobulin class switching and triggering of mouse NK cell-mediated cytotoxicity. 产品信息 格式 Purified 控制 - RAW264.7 cells
演示 Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Anti-CD86 Antibody, clone PO.3 is an antibody against CD86 for use in FC, IP, IH. 主要应用 - Immunohistochemistry
- Flow Cytometry
应用说明 Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Nuriya, S., et al., 1996).
Imunohistochemistry Analysis: A previous lot was used by an independent laboratory in IH (Nuriya, S., et al., 1996).生物信息 免疫原品种 Mouse CD86 cDNA-transfected P815 cells. 表位 Unknown 克隆 PO.3 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rat 特异性 This antibody recognizes CD86. 同种型 IgG2bκ 品种反应性 Mouse Species Reactivity Note Demonstrated to react with mouse. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_062261
基因符号 - LAB72
- CD86
- B7-2
- B70
- BU63
- CD28LG2
- FUN-1
纯化方法 Protein G purfied UniProt编号 - P42082
UniProt汇总 FUNCTION: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation.
SUBUNIT STRUCTURE: Interacts with MARCH8.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICTY: Expressed on activated B-cells.
PTM: Polyubiquitinated; which is promoted by MARCH8 and results in endocytosis and lysosomal degradation.
SEQUENCE SIMILARITIES: Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.产品使用声明 质量保证 Evaluated by Flow Cytometry in RAW264.7 cells.
Flow Cytometry Analysis: 1 µg of this antibody detected CD86 in RAW264.7 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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下一件merck millipore产品：merck millipore,默克密理博,PEZGS0816,Millicell EZ SLIDE 8-well glass, sterile
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