merck millipore,默克密理博,04-1007,Anti-FOXO3a Antibody, clone EP1949Y, rabbit monoclonal

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merck millipore,默克密理博,04-1007,Anti-FOXO3a Antibody, clone EP1949Y, rabbit monoclonal
产品名称：Anti-FOXO3a Antibody, clone EP1949Y, rabbit monoclonal
产品型号：04-1007
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

merck millipore,默克密理博,04-1007,Anti-FOXO3a Antibody, clone EP1949Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1007,Anti-FOXO3a Antibody, clone EP1949Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	ICC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1007
描述	Anti-FOXO3a Antibody, clone EP1949Y, rabbit monoclonal
Alternate Names	forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1 AF6q21 protein Forkhead in rhabdomyosarcoma-like 1 forkhead box O3 forkhead box O3A forkhead homolog (rhabdomyosarcoma) like 1
背景信息	Forkhead box O3A (FOXO3A) is a member of the forkhead family of transcription factors (FOXO), which play an important role in cellular proliferation, metabolism, stress tolerance, and aging. FOXO transcription factors have a characteristic forkhead domain and are located in the cytoplasm. Upon oxidative stress, FOXO3A translocates to the nucleus where binding to the DNA sequence 5’-[AG]TAAA[TC]A-3’ occurs and IGF-1 is dephosphlorated, resulting in apoptosis. In order for the cell to survive, IGF-1 must be phosphlorated by AKT1/PKB at Thr32 and Ser-253, which will result in retention and inactivation of FOXO3A in the cytoplasm. Translocation of FOXO3A with the MLL gene has been associated with secondary acute leukemia.

产品信息
格式	Unpurified
控制	Jurkat cell lysate
演示	Rabbit Monoclonal in buffer containing sodium azide, glycerol, and BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
主要应用	Immunocytochemistry Western Blotting
应用说明	Immunohistochemistry Analysis: A 1:50-100 dilution from a previous lot detected FOXO3a in human breast carcinoma tissue. Immunocytochemistry Analysis: A 1:50-100 dilution from a previous lot was used in IC.

生物信息
免疫原品种	Synthetic peptide corresponding to residues near the N-terminus of human FOXO3a.
表位	N-terminus
克隆	EP1949Y
宿主	Rabbit
特异性	This antibody recognizes FOXO3a at and around the N-terminus.
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, Mouse and Rat
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_001446
Entrez基因汇总	This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. This gene likely functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of this gene with the MLL gene is associated with secondary acute leukemia. Alternatively spliced transcript variants encoding the same protein have been observed.
基因符号	OTTHUMP00000016944 AF6q21 DKFZp781A0677 FKHRL1 FKHRL1P2 FOXO2 FOXO3A MGC12739 MGC31925
纯化方法	Unpurified
UniProt编号	O43524
UniProt汇总	FUNCTION: Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. SUBUNIT STURCTURE: Interacts with YWHAB/14-3-3-beta and YWHAZ/14-3-3-zeta, which are required for cytosolic sequestration. Upon oxidative stress, interacts with STK4, which disrupts interaction with YWHAB/14-3-3-beta and leads to nuclear translocation. SUBCELLULAR LOCATION: Cytoplasm › cytosol. Nucleus. Note: Translocates to the nucleus upon oxidative stress and in the absence of survival factors. TISSUE SPECIFICITY: Ubiquitous. PTM: In the presence of survival factors such as IGF-1, phosphorylated on Thr-32 and Ser-253 by AKT1/PKB. This phosphorylated form then interacts with 14-3-3 proteins and is retained in the cytoplasm. Survival factor withdrawal induces dephosphorylation and promotes translocation to the nucleus where the dephosphorylated protein induces transcription of target genes and triggers apoptosis. Although AKT1/PKB doesn't appear to phosphorylate Ser-315 directly, it may activate other kinases that trigger phosphorylation at this residue. Phosphorylated by STK4 on Ser-209 upon oxidative stress, which leads to dissociation from YWHAB/14-3-3-beta and nuclear translocation. INVOLVEMENT IN DISEASE: A chromosomal aberration involving FOXO3 is found in secondary acute leukemias. Translocation t(6;11)(q21;q23) with MLL/HRX. SEQUENCE SIMILARITIES: Contains 1 fork-head DNA-binding domain.

产品使用声明
质量保证	Evaluated by Western Blot on Jurkat cell lysates. Western Blot Analysis: A 1:2,000-5,000 dilution of this antibody was used to detect FOXO3a in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.