merck millipore,默克密理博,03-182,HDAC2 (1-488) (His-tag) human recombinant

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merck millipore,默克密理博,03-182,HDAC2 (1-488) (His-tag) human recombinant
产品名称：HDAC2 (1-488) (His-tag) human recombinant
产品型号：03-182
Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells.

merck millipore,默克密理博,03-182,HDAC2 (1-488) (His-tag) human recombinant

产品介绍

merck millipore,默克密理博,03-182,HDAC2 (1-488) (His-tag) human recombinant

重要规格表

主要应用	品种
EA	Human

描述
产品目录编号	03-182
品牌系列	Upstate
商名	Upstate
描述	HDAC2 (1-488) (His-tag) human recombinant
概述	Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells
背景信息	Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDAC2 is likely to be involved in regulating chromatin structure during transcription. It associates with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA. This process is highly conserved from yeast to human.

产品信息
格式	Purified
演示	In liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol

应用
应用	Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells.
主要应用	Enzyme Assay
应用说明	HDAC deacetylase assays

生物信息
纯度	Purity >50% by SDS-PAGE
来源	Residues 1-488 of human HDAC2
品种	Human
特异活性	>5 U/µg. 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity.
物理形式	Frozen solution

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC2 into separate tubes before storing at -80°C.

储存和货运信息

存储条件

Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC2 into separate tubes before storing at -80°C.