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merck millipore,默克密理博,03-180,RIPAb+™ G3BP1 - RIP Validated Antibody and Primer Set
产品名称：RIPAb+™ G3BP1 - RIP Validated Antibody and Primer Set
产品型号：03-180
This RIPAb+ G3BP1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers.
merck millipore,默克密理博,03-180,RIPAb+™ G3BP1 - RIP Validated Antibody and Primer Set
- 产品介绍
重要规格表
品种反应性 主要应用 H WB 描述 产品目录编号 03-180 商名 - Upstate
- RIPAb+
描述 RIPAb+™ G3BP1 - RIP Validated Antibody and Primer Set 概述 RIPAb+ antibodies are evaluated using the RNA Binding Protein Immunoprecipitation (RIP) assay. Each RIPAb+ antibody set includes a negative control antibody to ensure specificity of the RIP reaction and is verified for the co-immunoprecipitation of RNA associated specifically with the immunoprecipitated RNA binding protein of interest. Where appropriate, the RIPAb+ set also includes quantitative RT-PCR control primers (RIP Primers) to biologically validate your IP results by successfully co-precipitating the specific RNA targets, such as messenger RNAs. The qPCR protocol and primer sequences are provided, allowing researchers to validate RIP protocols when using the antibody in their experimental context. If a target specific assay is not provided, the RIPAb+ kit is validated using an automated microfluidics-based assay by enrichment of detectable RNA over control immunoprecipitation.
G3BP1 (Ras-GTPase activating protein SH3-domain binding protein 1) directly associates with SH3 domains of GTPase activating proteins (GAPs). G3BP1, along with G3BP2, has been shown to bind to the C-terminus of p53, playing a crucial role in its activity (Kim, 2007). Furthermore, the G3BPs have two traditional RNA binding motifs.Alternate Names - RasGAP-associated endoribonuclease G3BP
- ATP-dependent DNA helicase VIII
- GAP SH3 domain-binding protein 1
- GAP binding protein
- GTPase activating protein (SH3 domain) binding protein 1
- Ras-GTPase-activating protein SH3-domain-binding protein
背景信息 G3BP1 (Ras-GTPase activating protein SH3-domain binding protein 1) directly associates with SH3 domains of GTPase activating proteins (GAPs). It was first discovered by its binding to RasGAP. G3BPs have been shown to be involved in a number of mitogenic signaling pathways. G3BP1 is also known to be over expressed in many human cancers (Kim, 2007) and its expression appears to be inversely correlated with PTEN expression (Kim, 2007). Interestingly, G3BP1, along with G3BP2, has been shown to bind to the C-terminus of p53, playing a crucial role in its activity (Kim, 2007). Furthermore, the G3BPs have two traditional RNA binding motifs. 产品信息 格式 Purified 控制 - Includes negative control normal mouse IgG antibody and control primers specific for human c-myc 3' UTR.
演示 Anti-G3BP1 (mouse monoclonal),. One vial containing 50 µg of protein G purified mouse monoclonal IgG1ĸ in buffer containing 0.1 M Tris-glycine, 0.150 M NaCl, pH 7.4, and 0.05% sodium azide before the addition of 30% glycerol. Store at -20°C .
Normal Mouse IgG, . One vial containing 125 µg of mouse IgG in 125 µL of storage buffer containing 0.05% sodium azide. Store at -20°C.
RIP Primers, c-myc 3' UTR. One vial containing 75 μL of 5 μM of each primer specific for human c-myc 3' UTR. Store at -20°C.
FOR: CAC AAA CTT GAA CAG CTA CGG
REV: GGT GAT TGC TCA GGA CAT TTC
应用 应用 This RIPAb+ G3BP1 -RIP Validated Antibody & Primer Set conveniently includes the antibody & the specific control PCR primers. 主要应用 - Western Blotting
应用说明 Immunoprecipitation from RIP lysate:
Representative lot data.
RIP lysate from 293 cells (~2 X 10E7 cell equivalents per IP) was subjected to immunoprecipitation using 5 µg of either a normal mouse IgG, (Cat. #CS200621), or 5 µg of Anti-G3BP1 antibody (Cat. # CS204311). Ten percent of the precipitated proteins (lane 1: mouse IgG, lane 2: G3BP1) and 293 whole cell lysate (lane 3) were resolved by electrophoresis, transferred to nitrocellulose and probed with Anti-G3BP1 antibody (Cat. # CS204311, 1:1000). Proteins were visualized using One-Step™ IP-Western kit (GenScript Cat. # L00232)
Arrow indicates G3BP1.
Western Blot Analysis:
Representative lot data.
K562 cell lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with Anti-G3BP1 (0.1 ug/mL dilution).
Proteins were visualized using a Goat Anti-Mouse antibody conjugated to HRP using a chemiluminescence detection system.
Arrow indicates G3BP1 (~52 kDa).生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human G3BP1. 表位 Unknown 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes G3BP1. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Human. Other species have not yet been tested. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_005745.1
Entrez基因汇总 This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq] 基因符号 - HDH-VIII
- G3BP
- G3BP-1
纯化方法 Protein G purfied UniProt编号 - Q13283
UniProt汇总 FUNCTION: May be a regulated effector of stress granule assembly. Phosphorylation-dependent sequence-specific endoribonuclease in vitro. Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR. ATP- and magnesium-dependent helicase. Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends. Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency. Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA.
COFACTOR: Magnesium. Required for helicase activity.
SUBUNIT STRUCTURE: Binds to the SH3 domain of Ras GTPase-activating protein (RASA1) in proliferating cells. No interaction in quiescent cells Component of a TAU mRNP complex, at least composed of IGF2BP1, ELAVL4 and G3BP By similarity. Interacts with USP10, and may regulate it. Forms homodimers and oligomers.
SUBCELLULAR LOCATION: Cytoplasm. Cytoplasm › cytosol. Cell membrane. Nucleus. Note: Cytoplasmic in proliferating cells, can be recruited to the plasma membrane in exponentially growing cells By similarity. Cytosolic and partially nuclear in resting cells. Recruited to stress granules (SGs) upon either arsenite or high temperature treatment. Recruitment to SGs is influenced by HRAS.
TISSUE SPECIFICITY: Ubiquitous.
DOMAIN: The NTF2 domain mediates multimerization.
PTM: Phosphorylated exclusively on serine residues. Hyperphosphorylated in quiescent fibroblasts. Hypophosphorylation leads to a decrease in endoribonuclease activity By similarity. RASA1-dependent phosphorylation of Ser-149 induces a conformational change that prevents self-association. Dephosphorylation after HRAS activation is required for stress granule assembly. Ser-149 phosphorylation induces partial nuclear localization.
Arg-435 is dimethylated, probably to asymmetric dimethylarginine.
SEQUENCE SIMILARITIES: Contains 1 NTF2 domain.
Contains 1 RRM (RNA recognition motif) domain.产品使用声明 质量保证 RNA Binding Protein Immunoprecipitation:
Representative lot data.
RIP Lysate prepared from 293 cells (~2 X 10E7 cell equivalents per IP) were subjected to immunoprecipitation using 5 µg of either a normal mouse IgG , or 5 µg Anti-G3BP1 antibody and the Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Cat. # 17-700).
Successful immunoprecipitation of G3BP1-associated RNA was verified by qPCR using RIP Primers, c-myc 3' UTR, (Figure 1).
Please refer to the Magna RIP™ (Cat. # 17-700) or EZ-Magna RIP™ (Cat. # 17-701) protocol for experimental details.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
包装信息 数量 10 assays 包装 10 assays per set. Recommended use: ~5 μg of antibody per RIP (dependent upon biological context).
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